This research aims to establish the effects of different heat treatment temperatures applied to turkey sausages on the physicochemical, microbiological, and textural features of the product and biogenic amine formation during heat treatment and storage. To this end, three different internal temperatures were applied to turkey sausages fermented for 3 days at 23oC and relative humidity (85%±1). The product’s lactic acid bacteria (LAB), Micrococcus/Staphylococcus growth, pH, residual nitrite, 2-thiobarbituric acid reactive substances (TBARS) and biogenic amine (tyramine, histamine, putrescine, cadaverine, spermidine and spermine) formation were analyzed during heat treatment and storage (18 oC, 20 days). Different internal temperature applications affected LAB growth, residual nitrite, and biogenic amine formation (P<0.05). While LAB, Micrococcus/Staphylococcus growth and residual nitrite values decreased significantly during storage (P<0.05), the amounts of biogenic amines, other than spermidine, increased. Furthermore, no difference was detected in pH and TBARS values (P> 0.05). The interaction of different internal temperature applications and storage times affected only LAB and biogenic amine amounts. As a result of the study, increasing internal temperature applications caused significant changes in the quality characteristics of turkey sausage during the storage period. Although the LAB values decreased with the increase in the internal temperatures, the biogenic amine amounts during storage increased with increasing internal temperature application. Therefore, heat treatment at 62oC for 5 min is the most suitable method to be used for turkey sausage.