Fungi associated with Vaccinium species play important roles in plant growth and disease control, especially in the final blueberry production. Vaccinium dunalianum var. urophyllum (Ericaceae) is a well-known medicinal plant in Southern China used to treat inflammation and microbial infections. The endophytic fungi from these plants are therefore anticipated as potential new sources of antimicrobials. In this report, the inhibitory effects of endophytes against clinical bacteria and yeast were comprehensively screened and 11 isolates indicated high bioactivity by the agar diffusion method. The corresponding crude extracts of these fungi under submerged fermentation also demonstrated distinct differences and n-butyl alcohol displayed the lowest extraction efficiency among the extracts. The ethyl acetate and dichloromethane extracts of filtrates from the Colletotrichum sp. VD001, Epicoccum nigrum VD021 and E. nigrum VD022 strains displayed good properties against pathogenic microorganisms according to disc diffusion assays and minimal inhibitory concentration (MIC). This study is the first indicating that cultivable endophytic fungi associated with blueberry plants produce potential compounds against clinical pathogens. ABSTRAKKulat yang dikaitkan dengan spesies Vaccinium memainkan peranan penting dalam pertumbuhan tumbuhan dan kawalan penyakit, terutamanya dalam pengeluaran beri biru akhir. Vaccinium dunalianum var. urophyllum (Ericaceae) ialah tumbuhan ubatan yang terkenal di Selatan China yang digunakan untuk merawat jangkitan keradangan dan mikrob. Oleh itu, kulat endofit daripada tumbuhan ini dijangka sebagai sumber baru antimikrob yang berpotensi. Dalam laporan ini, kesan perencatan endofit terhadap bakteria klinikal dan suatu ragi telah ditayangkan secara komprehensif dan 11 pencilan menunjukkan bioaktiviti tinggi melalui kaedah penyebaran agar. Ekstrak mentah yang sepadan dengan kulat ini di bawah penapaian terendam juga menunjukkan perbezaan yang ketara serta n-butil alkohol menunjukkan kecekapan pengekstrakan terendah dalam kalangan ekstrak. Ekstrak etil asetat dan diklorometana filtrat daripada Colletotrichum sp. VD001, Epicoccum nigrum VD021 dan E. nigrum VD022 menunjukkan sifat-sifat yang baik terhadap mikroorganisma patogen berdasarkan ujian penyebaran cakera dan kepekatan perencatan minimum (MIC). Kajian ini adalah kajian yang pertama menunjukkan bahawa kulat endofit yang boleh dikaitkan dengan tumbuhan beri biru menghasilkan sebatian yang berpotensi terhadap patogen klinikal.Kata kunci: Aktiviti antimikrob; kepekatan perencatan minimum; kulat endofit; spesies Vaccinium
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