Highlights d Analysis of genome-wide data for nine sites from the Bronze Age Southern Levant d Contemporaneous samples from multiple sites are genetically similar d Migration from the Zagros and/or Caucasus to the Levant between 2500-1000 BCE d People related to these individuals contributed to all presentday Levantine populations

Vast burial fields, some with hundreds of burials, categorize the southern Levant’s Intermediate Bronze Age period (IBA). This phenomenon contrasts with a limited number of burials found from the preceding Early Bronze III period. This paper presents the first radiocarbon dating research of sampled bones from shaft tombs from five IBA burial sites across Israel: Yehud, Jebel Qaaqir, Sheikh-Danon, Hazorea, and Kefar-Veradim. Prescreening methods, including Fourier transform infrared analysis, were applied to identify best-preserved collagen in archaeological bones for radiocarbon dating. Overall, the measured date ranges cover the IBA timeline, supporting the observation that the IBA signature shaft tombs are a fundamental tradition of the IBA culture, at least in Israel. A single IBA shaft tomb at Jebel Qaaqir which contained remains of multiple humans, supplied different dates for various people, spanning over a few hundred years. These results suggest a tribal or family-oriented IBA community with a long-lasting tradition reflected in centuries of collective burial practices.