ABSTRACT. The aim of this study was to evaluate the effect of a commercial formulation of Methomyl on the reproduction in Daphnia obtusa. Neonates (<24 h) were exposed to concentrations of insecticide (1.0, 1.5, 2.0, 2.5, 3.0 µg L -1 ) for a period of 21 days. The results showed a significant decrease in the number of molts, total neonates, fertility and sex ratio index. Significant regressions were found between reproductive parameters and concentrations of the insecticide: molts (R 2 = 0.96), fertility (R 2 = 0.97), number of female neonates born per female (R 2 = 0.90), and the number of male neonates born per female (R 2 = 0.94). Embryotoxicity was also observed; the number of neonates born with malformations increased in the highest concentrations of Methomyl 90 SP® tested. In conclusion, Methomyl 90 SP® inhibits reproduction of D. obtusa. However, a clear effect as an endocrine disrupter was not observed. This insecticide is embryotoxic; it causes malformations in neonates of Daphnia obtusa. Keyswords: Daphnia, endocrine, neonates, pesticide, disruptor, zooplankton, ecotoxicology. Evaluación del efecto de una formulación comercial de Metomil sobre la reproducción de Daphnia obtusa Kürz (1874)RESUMEN. El objetivo de este trabajo fue evaluar el efecto de una formulación comercial de Metomil sobre la reproducción en Daphnia obtusa. Para ello, neonatos de D. obtusa (<24 h) fueron expuestos a concentraciones del insecticida (1.0, 1.5, 2.0, 2.5, 3.0 µg L -1 ) por un período de 21 días. Los resultados mostraron una disminución significativa en el número de mudas, recién nacidos totales, fertilidad e índice de proporción de sexos. Se encontraron regresiones significativas entre algunos parámetros reproductivos y las concentraciones del insecticida: mudas (R 2 = 0.96), fertilidad (R 2 = 0.97), número de neonatos hembras por hembra (R 2 = 0.90) y el número de recién nacidos machos (R 2 = 0.94). También se observó embriotoxicidad, el número de neonatos nacidos con malformaciones aumentó en las concentraciones más altas de Metomil 90 SP® probadas. En conclusión, el Metomil inhibe la reproducción de D. obtusa, aunque no se observó en forma clara un efecto como disruptor endocrino. Este insecticida es embriotóxico, ya que provoca malformaciones en los neonatos de Daphnia obtusa.
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