In tritrophic 'plants-herbivores-natural enemies' systems, there are relatively few reports concerning the role(s) of kairomones in pupal parasitism. Chouioia cunea Yang (Hymenoptera: Eulophidae), an endoparasitic chalcid wasp, parasitizes pupae of the fall webworm (Hyphantria cunea Drury). The role of host-related kairomones was investigated using electroantennogram (EAG) and behavioral techniques. Chemicals from some host stages (pupae) and host by-products (frass), induced arrestment behavior of female parasitoids, while chemicals from prepupae, were inactive. Gas chromatography-mass spectrometry analysis of volatiles collected from pupae, frass and prepupae using solid-phase microextration revealed seven compounds with carbon chain lengths ranging from C4 to C20. All of the chemicals elicited significant EAG responses in C. cunea. Y-tube olfactometer bioassays demonstrated a significant positive response of mated female C. cunea to 1-dodecene. These data provide a better understanding of the host location mechanisms of pupal parasitoid.
Two new species of the genus Cratospila Foerster, 1863 (Braconidae: Alysiinae), Cratospila albosignatasp. nov. and C. longivenasp. nov., are described and illustrated. In addition, the DNA barcode region of the mitochondrial cytochrome c oxidase subunit I (COI) of both species has been sequenced with three previously described species (C. albifera, C. luteocephala and C. syntoma). Alysia ponerola Papp, 2009 which was recorded from North Korea is transfered in Cratospila (C. ponerola (Papp, 2009) comb. nov.). All species validly recorded from Korea are included in a revised key.
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