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Background: Phenol compounds and flavonoids are known have antioxidant activity. Sterculia genus has secondary metabolite rich of phenols and flavonoids. Objective: The aim of this study of the activity antioxidants of Sterculia stipulata Korth. Woods and leaves by FRAP method. Materials and methods: Extraction done using n-hexane, ethyl acetate, and methanol. The methanol extract was determined antioxidant activity using the FRAP method and also determined the total phenols content, total flavonoids, and phytochemical screening. Results: The antioxidant activity of wood extract was 4.74 ± 1.03 FeEAC (mol/g) while leaves extract 41.17 ± 1.99 FeEAC (mol/g). Total phenols content for wood extract 16.46 ± 3.51 mg GAE/g, for leaves extract 141.62 ± 10.54 mg GAE/g. The total flavonoids content for woods extract was 27.99 ± 0.62 mg QE/g for leaf extract 41.45 ± 5.83 mg QE/g. The compounds of woods and leaves are the same; it is consist of terpenoids, alkaloids, phenols, flavonoids, saponins, terpenoids, and negatives for anthraquinone. Conclusion: The antioxidant activity of the leaves of Sterculia stipulata Korth. is greater than its wood activities.

Elastase is a proteinase enzyme that can reduce elastin by dividing specific peptide bonds. Therefore, the inhibition of elastase activity in the dermis layer can be used to maintain skin elasticity. Materials that can inhibit elastase activity can be a cosmetic ingredient in dealing with skin aging. Increased desire to maintain healthy skin without chemicals, encourages the use of materials from natural sources such as plants from Indonesia, a country that has high biodiversity, including Garcinia latissima Miq. from the Clusiaceae family. The purpose of this study was to explore and sought an explanation as to why G. latissima Miq. inhibits elastase enzyme. Plant extraction was carried out using maceration successively methods. The inhibition activity of elastase enzyme was carried out by measuring the kinetic enzyme N-succ-(Ala)-3-Nitroanilide conversion to p-nitroaniline (substrate) spectrophotometry at 405 nm and using porcine pancreatic elastase (PPE) as an enzyme. The results showed that the G. latissima Miq. methanol extract and ethyl acetate extract were active as elastase enzyme inhibitors. G. latissima Miq. extract can maintain skin elasticity.

Rubus fraxinifolius can be founded in the mountainous regions of West Java. Previous studies have shown that this plant has potent antioxidant activity. The content of polyphenol compounds in this species contributes substantially to antioxidant activity, and the extraction method will aff ect the content of the compound. In this study, an evaluation of the extraction method eff ect on leaves, young fruits, and stems of R. fraxinifolius on the content of polyphenols and their antioxidant activity, have been done. The leaves, fruits, and stems of R. fraxinifolius were extracted by maceration, refl ux, and Soxhlet using methanol as a solvent. Each extract was determined the total polyphenol content using Folin-Ciocalteu reagent and antioxidant activity test using DPPH reduction method. The highest extract yield was R. fraxinifolius leaf refl ux extract (30.20%). The highest total polyphenol content was given by the Soxhlet extract of leaf R. fraxinifolius (48.79 mg GAE/g extract). The highest antioxidant activity was leaf Soxhlet extract of R. fraxinifolius (98.29% at 100 ppm). From this study it was shown that the methanol extract of R. fraxinifolius leaves extracted by soxhlet gave the highest polyphenol content and antioxidant activity.Abstrak: Tanaman Rubus fraxinifolius dapat ditemukan di daerah pegunungan Jawa Barat. Penelitian sebelumnya menunjukkan bahwa tanaman ini memiliki aktivitas antioksidan yang sangat kuat. Kandungan senyawa polifenol pada tanaman berkontribusi secara substansial terhadap aktivitas antioksidan dan metode ekstraksi akan mempengaruhi kandungan senyawa yang tersari. Pada penelitian ini dilakukan evaluasi pengaruh metode ekstraksi organ daun, buah muda, dan batang R. fraxinifolius terhadap kandungan polifenol serta aktivitas antioksidannya. Bagian daun, buah, dan batang R. fraxinifolius diekstraksi cair padat secara maserasi, refl uks, dan Soxhlet menggunakan pelarut metanol. Terhadap masing-masing ekstrak dilakukan penetapan kadar polifenol total dengan pereaksi Folin-Ciocalteu serta uji aktivitas antioksidan dengan metode peredaman DPPH. Rendemen ekstrak terbanyak adalah ekstrak refl uks daun R. fraxinifolius sebesar 30,20%. Kandungan polifenol total tertinggi adalah pada ekstrak Soxhlet daun R. fraxinifolius sebesar 48,79 mg GAE/g ekstrak. Aktivitas antioksidan tertinggi yaitu ekstrak Soxhlet daun R. fraxinifolius sebesar 98,29% pada 100 bpj. Dari penelitian ini menunjukan bahwa ekstrak metanol daun R. fraxinifolius yang diekstraksi secara Soxhlet memiliki kandungan polifenol serta aktivitas antioksidan tertinggi.Kata kunci: Rubus fraxinifolius, metode ekstraksi, rendemen, polifenolat, antioksidan.