Sexual dimorphism in flies has been evidenced in classical morphological characters, but little is known about the differentiation in wing geometry, a multivariate character highly controlled at the genetic level that influences the flight and impacts its life history and can be analyzed through geometric morphometrics. Here, we evaluated sexual dimorphism in the wing geometry of Musca domestica L., a cosmopolitan synanthropic fly. Specimens from Colombia were used for wing mounting, photography, and landmark-based geometric morphometric analysis. We found significant differences between male and female specimens in wing size and shape. Particularly, cross-validated classification based on wing shape resulted in high scores (90%) of correct sex attribution. The influence of wing geometry differences on the flight performance and life history in this species remains unknown.

In this work, the wasp Brachymeria podagrica is recorded for the first time for Colombia, a solitary parasitoid of larvae of the Peckia collusor fly. Third instar larvae of P. collusor were exposed to outdoor ambient conditions for 6 hours in the garden of the Tecnológico de Antioquia (Medellín, Colombia). 29 B. podagrica adults were obtained from the host pupae. Prevalence of parasitism was 14.5 %.

The genus Fannia Robineau-Desvoidy is a group of calyptrate Diptera distributed in all biogeographic regions and comprises about 300 species. The present study described five new species from Colombia: Fannia aburrae sp. nov., Fannia copacabana sp. nov., Fannia isa sp. nov., Fannia pseudoconstricta sp. nov., Fannia spinosa sp. nov. Male specimens are described, and morphological characters and terminalia are illustrated. Likewise, an updated identification key for males and an updated checklist for the 37 species present in Colombia are provided. New records are presented for the country.

La obtención de ADN de moscas de interés médico-legal es de relevancia para una variedad de aplicaciones. Aunque existen métodos de extracción comerciales de ADN, su uso rutinario es limitado, en algunos escenarios. En este contexto, el uso de métodos no comerciales constituye una alternativa; sin embargo, su optimización es clave para mejorar el flujo de trabajo y los resultados. Este trabajo evaluó el impacto de variaciones a un método de precipitación salina sobre la concentración y la pureza del ADN recuperado. No se encontraron diferencias significativas en la concentración de ADN extraído entre los diferentes tiempos de incubación, probados durante la fase de extracción, mientras que el incremento en el volumen de etanol absoluto, en la fase de precipitación de ADN, mejoró significativamente la concentración de ADN obtenido. Las modificaciones propuestas reducen el tiempo de ejecución y la concentración de ADN obtenido comparado con el protocolo original.

Fannia Robineau-Desvoidy is a genus of calyptrate Diptera that comprises 89 Neotropical species, of which only 23 occur in Colombia. Based on male characters (including terminalia), two new species from the Department of Antioquia (Fannia colazorrensis sp. nov. and Fannia laclara sp. nov.) are described. Illustrations of the male are presented.