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BACKGROUND: Infection is the most common diseases in developing country, including Indonesia. Bacteria that often causes infection is Escherichia coli and Staphylococcus aureus. One of the traditional plants that can be used as an antibacterial is lidah mertua. AIM: The purpose of this study was to find out the profile of chemical compounds by thin layer chromatography method and determine the antibacterial activity of Lidah Mertua leaves by in vitro. METHODS: This research conducted an experimentally using non-polar, semipolar, and polar as solvents to get extract against E. coli and S. aureus as bacterial testing. The antibacterial activity using agar diffusion method to get minimum inhibitory concentration (MIC). RESULTS: The result of the research on thin layer chromatography showed that the compounds contained in the Lidah Mertua leaves were polifenol, steroids and alkaloids. The data obtained were tabulated and analysed descriptively. The antibacterial activity show that n-hexane extract does not provide inhibitory activity. MIC value show that aethyl acetate extract of lidah mertua leaves inhibited the growth of E. coli and S. aureus at concentration 50 mg/mL and 25 mg/mL with diameters of inhibition zone is 8.50 mm and 8.20 mm and methanol extract of lidah mertua leaves inhibited the growth of E. coli and S. aureus at concentration 12.5 mg/mL and 25 mg/mL with diameters of inhibition zone is 8.46 mm and 8.32 mm. CONCLUSION: The profile of chemical compounds by thin layer chromatography method showed that the compounds contained in the Lidah Mertua leaves were polifenol, steroids and alkaloids. The antibacterial activity show that n-hexane extract does not provide inhibitory activity, but aethyl acetate extract of lidah mertua leaves inhibited the growth of E. coli and S. aureus.

Telah dilakukan pengujian efektivitas antelmintik ekstrak etanol biji pinang (Areca Catechu L.). Pengujian dilakukan secara in vitro dengan hewan uji Cacing Ascaridia galli yang direndam di dalam larutan ektrak biji pinang, dengan pembanding piperazin sitrat 0,2%. Hewan uji dibagi dalam lima kelompok masing-masing terdiri dari lima 5 ekor cacing Ascaridia galli . Kelompok I, II, III, adalah kelompok uji ekstrak biji pinang pada konsentrasi 1%, 2%, 4%. Kelompok IV, adalah pembanding piperazin sitrat pada konsentrasi 0,2%, sedangkan kelompok kontrol negatif menggunakan NaCl 0,9%. Data yang dikumpulkan adalah jumlah total cacing yang mati pada setiap perlakuan selama 24 jam. Dari hasil penelitian menunjukkan bahwa ekstrak etanol biji pinang (Areca catechu L.) memiliki efek antelmintik pada semua konsentrasi dan yang mempunyai efek antelmintik yang paling baik dengan jumlah cacing yang mati sebanyak 11 cacing dari 15 sampel yang digunakan adalah ekstrak etanol biji pinang pada konsentrasi 4%, namun tidak lebih baik dari jumlah cacing yang mati pada piperazin sitrat sebanyak 15 cacing dari 15 sampel.

Red betel leaf (Piper crocatum Ruiz & Pav.) is a plant that has medicinal and cosmetic properties. One of the cosmetics used by society today is facial serum. The purpose of this study was the formulation of facial serum from red betel leaf water extract. The water extract was obtained by means of the Pressurized Hot Water Extraction method using distilled water as a solvent, then phytochemical screening was carried out. Serum is made in several concentrations, namely 1.5% (F1); 2% (F2);3%;(F3) and blank (F0). The quality inspection of the preparation includes homogeneity test, pH, viscosity, stability, irritation test, hedonic test, and antioxidant activity test. The results of phytochemical screening of red betel leaf water extract contained alkaloids, flavonoids, tannins, saponins and triterpenoids. Red betel leaf is formulated into a homogeneous serum preparation with a temporary pH range of 6.1-6.2 after testing has a pH of 5.8-6.1 and does not irritate the skin. The results of the antioxidant activity test showed that the water extract of red betel leaf (Piper crocatum Ruiz & Pav.) was included in the "strong" antioxidant category with an IC50 value of 38.30 g/ml. The IC50 values ​​for serum preparations F0, F1, F2 and F3 were 430.3689 g/ml (weak), 252.03 g/ml (moderate), 115.6 g/ml (moderate) and 58.13 g/ml (strong). Based on this study, it was concluded that all facial serum formulas were homogeneous, stable and did not irritate the skin and serum preparations with 3% red betel leaf water extract (F3) had the best activity in the category of strong antioxidants.

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