Introduction: Platelet rich plasma (PRP) is an autologous serum that contains high concentrations of platelets and growth factors. It is currently a versatile therapy in dermatology; however, few studies objectively evaluate its efficacy. Objective: To evaluate the efficacy and safety of intradermal microinjection of autologous platelet concentrate (APC) in the treatment of crow's feet rejuvenation. Method: An observational, analytical and longitudinal study was carried out in 60 patients from the Hospital Clínico Quirúrgico: “Hermanos Ameijeiras”, in the period between March 1, 2017 and March 31, 2020. The treatment was applied monthly. for 1 year. The final evaluation was carried out 3 months after the end of the treatment. Results: 60 women with an average age of 45 ± 4.3 years were treated. After treatment, there were significant changes in the Glogau Photo Damage Scale (P = 0.0022), in the Photonumeric Scale to assess the severity of crow's feet (P = 0.0111) and in the Global Aesthetic Improvement Scale. (P = 0.0033). The adverse events found were pain, inflammation and ecchymosis. The degree of satisfaction reported by the patients was good (16.6%) and very good (83.3%) (P = 0.0021). Conclusions: The autologous platelet concentrate proved to be effective and safe in reducing the signs of cutaneous aging of crow's feet, associated with a high degree of patient satisfaction.