Background Circular RNAs (circRNAs) are crucial in the regulation of gene expression and biological processes. However, in colorectal cancer, the expression characteristics and biological function of circRNA_0006174 (circ_0006174) is not fully understood. This work is aimed to investigate the biological function of circ_0006174 in colorectal cancer and its molecular mechanism. Methods Circ_0006174, microRNA-142-3p and X-linked inhibitor of apoptosis expression levels were detected in colorectal cancer tissues and cells using quantitative real-time polymerase chain reaction analysis or Western blot. The effects of circ_0006174 on colorectal cancer cell proliferation, apoptosis, migration and invasion were detected using the cell counting kit-8 method, bromodeoxyuridine experiments, flow cytometry analysis and Transwell experiments. The targeting relationship among circ_0006174, microRNA-142-3p and X-linked inhibitor of apoptosis was analysed by bioinformatics prediction, dual-luciferase reporter experiment and RNA immunoprecipitation experiment. Results Circ_0006174 was up-regulated in colorectal cancer tissues as well as in cell lines, and its high expression was remarkably associated with enlarged tumour volume and advanced tumour, node, metastasis stage of the patients. Circ_0006174 overexpression enhanced colorectal cancer cell proliferation, migration and invasion, and inhibited colorectal cancer cell apoptosis; while knocking down circ_0006174 caused the opposite effects. Circ_0006174 directly targeted and negatively regulated microRNA-142-3p expression, and X-linked inhibitor of apoptosis, a target gene of microRNA-142-3p, could be indirectly and positively modulated by circ_0006174. Conclusion Circ_0006174 facilitates colorectal cancer cell proliferation, migration and invasion, and represses colorectal cancer cell apoptosis by regulating microRNA-142-3p/X-linked inhibitor of apoptosis axis.

Source of materialDropwise addition of 1.0 ml of Na2CO3 (1 Msolution) to aaqueous solution of CuCl 2.·.2H2O( 0.170 g, 1.0 mmol) in 5.0 ml of H 2 Ogave ablue precipitate, which was separated by centrifugation and washed with water until no Cl anions were detectable in the supernatant. The collected blue precipitate was transferred to amixture solution of ethanol and water (1:1 v/v,10ml), to which 1,10-phenanthroline (phen, 0.198 g, 1.00 mmol) and phenyl-acetic acid (0.136 g, 1.00 mmol) were added successively. The resulting blue solution (pH =7.82) was allowed to stand at room temperature. Blue block-like crystals were grown by slow evaporation for over 7days. DiscussionThe crystal structure of the title compound is composed of a dinuclear cation [Cu(C 12H8N2)(OH)(H2O)]2 2+ ,a na nion (C 8 H 7 O 2 ) 2 2-and six lattice water molecules. The local coordination of copper(II) ion is tetragonal pyramidal. Two nitrogen atoms of phen and two hydroxyl oxygen atoms are located in the equatorial plane of the tetragonal pyramid with 2+ units indicate significant p-p stacking interactions making acontribution to formation of two-dimensional layers with the interplanner distance of neighboring aromatic rings is 3.17 Å.Inthe phenylacetate anion, two oxygen atoms are in acommon plane and six carbon atoms reside in the other plane, the dihedral angle between the being 26.5°. Through extensive hydrogen bonds between the carboxylate oxygen atoms of free phenyl-acetate, m 2 -OH-, guest and coordination water molecules, along with p-p stacking interactions, title compound exhibits at hree-dimensional supramolecular framework.

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