Materials Chloramphenicol (BioBasic ©), hydroxypropyl methylcellulose (HPMC) (Colorcon ®), tween 80 (Brataco ®), propylene glycol (Brataco ®), methyl paraben (Merck ®), glycerin (Brataco ®), potassium dihydrogen phosphate (Merck ®), sodium hydroxide (Merck ®), Fluid Thioglycollate Media (Merck ®), Soybean Casein Digest (TSB) (Merck ®), Nutrient Agar (Merck ®), cellophane membrane (Sigma ©), Escherichia coli (E.coli) (29998ATCC), ethanol (Brataco ®), and aqua bidestilata sterile (Ikapharmindo ®). Methods The experimental laboratory was conducted in stages. Preformulation Examination of the active substances used (chloramphenicol) by the monograph of the Indonesian Pharmacopoeia were: 14

Increasing public demand for cosmetics, especially those derived from natural materials, has provided seaweed opportunities as raw material for cosmetics. Kappa and iota carrageenans produced from red seaweed (Rhodophyceae) be used as a thickener and stabilizer in lotion formulations. This study aimed to determine the characteristics of lotion oil/water (o/w) type using kappa and iota carrageenan in the formulations. The research started with the optimization of lotion formulas. Four formulas obtained were 0% carrageenan (without carrageenan), 0.2% of kappa-carrageenan, 0.2% of iota-carrageenan, and a mixture of 0.2% kappa-carrageenan and 0.1% iota-carrageenan (2:1). Parameters observed were the organoleptic test, pH, viscosity, spreadability, microbial contamination, and a hedonic test. The results showed that kappa and iota carrageenan could be used to formulate lotion o/w type with a concentration of ⩽0.2%. The best formulation that meets the Indonesian National Standard (SNI) based on physical evaluation and hedonic test (parameters of moisture and sticky feeling) was the lotion formula with 0.2% of iota carrageenan.

Free radicals are naturally produced from the body's metabolic processes, but the excessive amount of free radicals can interfere with human health because they cause oxidative stress. Therefore we need antioxidants that can protect against free radicals. Ficus lyrata W. is one of the antioxidant sources. This study aims to formulate instant granules from the Ethanol extract of Ficus lyrata W. using the wet granulation method. The formula is optimized using the Design Expert with the two-level factorial method. The optimized factors are xanthan gum 0,8-1,5% and PVP 0,5-5%. Granules are evaluated and analyzed using a Design Expert. The results obtained showed that Formula 4 with a combination of xanthan gum 0,8% and PVP 5% is the best formula, which the evaluation result is Loss On Drying (LOD) 3,28%, Flowability 16.043 ± 0.221 (g/s), Angle of Rest 21.77 ± 0.862, no precipitate for15 minutes, pH = 4.7, dispersed in 31 seconds and sedimentation time is 52.213 ± 1.7878 (minutes), the results of the antioxidant activity test of the ethanol extract of Ficus lyrata is 38,27 µg/ml, and instant granules is 145,02 µg/ml.Keywords: Antioxidant, Ficus lyrata W., Instant granules, Design expert