A Sawback angelshark (Squatina aculeata) classified as “Critically Endangered” Global Red List by the IUCN (2017) and CITES (2017) is one of the threated elasmobranch groups and the increased pollution in the marine environment negatively impact angelshark species. Despite this, limited information exists about the effects of toxic chemical contamination in angelsharks. Therefore, present study was conducted to elucidate heavy metal accumulation in Squatina aculeata captured from the Iskenderun Bay, North-Eastern Mediterranean by electrochemical technique. Arsenic (As), Mercury (Hg), Copper (Cu), Iron (Fe), Zinc (Zn) and Mangan (Mn) were found by electrochemical analyzer. These results showed that Fe and Zn were the highest concentrations in all tissues of S. aculeata and, the accumulations of Hg, Fe, Zn and Mn were significantly different in the gill, liver and muscle tissues (P < 0.05). As and Hg concentrations exceeded maximum limit legalized by FAO (2003) and UNEP (2008). This results confirmed that the metal contamination in North-Eastern Mediterranean area may pose a potential threat to the sustainability of S. aculeata in marine ecosystem