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Cucumber is one of the most important vegetable crops in the world. To establish an efficient genetic transformation system in cucumber, the role of different factors such as pre-cultivation time, acetosyringone concentration, infection time and cocultivation time that can influence the transformation, was evaluated. In addition, Csexpansin 10 (CsEXP10) gene was transformed into the cucumber genome to produce transgenic lines. The results showed that various optimal parameters such as 100 mg L -1 of kanamycin concentration for selection of transformants, 2 d of pre-cultivation time, 100 μmol L -1 of acetosyringone concentration, 15 min of infection time and 2 d of co-cultivation time were obtained using cotyledonary node explants. The rooting frequency observed on Murashige and Skoog (MS) medium supplemented with 0.2 mg L -1 indole acetic acid and 400 mg L -1 cefotaxime was found to be 100.00%. The positive transgenic cucumber lines were identified using PCR analysis and GUS staining assay. It suggests that the genetic transformation system developed using cotyledonary node explants is efficient and successful in cucumber.

Chinese chive is an important leafy vegetable, for which nitrogen is vital for growth, development and nutritional quality. In order to study the effects of improved hoagland nutrient solution with different nitrate (NO 3 -) to ammonium (NH 4 + ) ratios (25:75, 50:50, 75:25 and 100:0) on the contents of chlorophyll and mineral elements (Ca, Fe, Zn, Cu, Mn, Mg and K) for three cultivars, Saisong, Pingjiu No.4 and Pingjiu No.8, under 14 mmol/L nitrogen. An experiment was conducted at experimental plots and lab of the school of horticulture and landscape architecture, henan institute of science and technology. The results showed that the chlorophyll a, chlorophyll b and total chlorophyll of the three cultivars were all reached a maximum value with NO 3 and NH 4 + ratio of 50:50. The eight mineral elements contents were higher for three cultivars at a NO 3 and NH 4 + ratios of 50:50 and 75:25. It could be concluded that the NO 3 and NH 4 + ratio of 50:50 effectively improved the chlorophyll contents and promoted accumulation of mineral elements in Chinese chive under hydroponic culture and was the optimal ratio.

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