An azobenzene-based heteromeric prodrug (hNDP) was prepared for targeted chemotherapy against hypoxic tumor. hNDP could divert the parent drug from nucleus to cytoplasm with lower toxicity, while the azoreduction of hNDP in hypoxia would activate the drug with a robust anti-tumor effect by initiating the apoptosis-related biochemical cascades.

activity of FNDs can reduce oxidative stress damage to astrocyte cells. More interestingly, the level of ROS, the content of MDA, the LDH leakage rate, and the expression of keap1 mRNA and protein in astrocytes from a mouse model of epilepsy seizures were decreased. On the contrary, the content of SOD, GSH, and the expression of Nrf2, HO-1 protein, and mRNA was significantly increased after incubation with FNDs. Therefore, FNDs can protect epileptic astrocytes by reducing oxidative stress through positively regulating the Nrf2/ARE pathway, inhibiting the production of free radicals and lipid peroxidation products to reduce oxidative stress. Collectively, our FNDs with minimal cytotoxicity can efficiently track their locations and protect mouse astrocytes from oxidative stress, which may provide a nano-based biomedicine platform for treating epilepsy-like neurological diseases.

A large-format manufacturing process of electric heating floors for indoor heating was evaluated in this work using large-format high-density fiberboard as the substrate, carbon fiber paper as the electric heating element, and semi-cured melamine resin film as the bonding material to elevate its production efficiency. The semi-cured melamine resin film permeated the carbon fiber paper to form a glued structure via hot-pressing process, which improved the water resistance and insulation of the electric heating layer. The internal bonding strength of the floor reached 1.63 MPa with the enhanced waterproof properties. The temperature rise of both the electric heating floor and the assembled floors can reach 20.5 °C within 60 min under the voltage of 220 V. There was a small difference of approximately 2 °C in the ambient temperature between the heights of 0.6 m and 1.8 m above the running assembled electric heating floors. The power of the assembled 16 pieces of floors stabilized at 830 W after the continuous electrification for 12 h in this test condition, which demonstrated the comfortable and high-efficiency heating performance of the assembled electric heating floors.

In this study, the efficacy of phycocyanin in bone defect repairing was tested on a zebrafish skull defect model, and the evaluating process was monitored in vivo using optical coherence tomography (OCT). Thirty zebrafish were randomly divided into three groups, which were immersed in water and phycocyanin solution (50 and 100 mg/L) after skull defect creating. All zebrafish were examined by OCT immediately after craniotomy, and on the 10th and 20th days of phycocyanin treatment. All the model fish were euthanized to enable a histological evaluation of skull after 20 days of recovery. OCT images demonstrated that phycocyanin (50 mg/L) could repair a cranial defect within 20 days. A high concentration (100 mg/L) of phycocyanin may favor the recovery of bone abnormalities in 10 days, but with the extended treatment time to 20 days, a deformation of the skull occurs.

Zebrafish is widely used in bone research, but there is a lack of methods to assess its bone development in vivo. The objective of this study was to monitor skull growth of zebrafish and investigate whether a correlation exists between the bone thickness and body length and age. The skull of zebrafish with different body lengths or ages were evaluated using optical coherence tomography (OCT) and histology. There was a significant normal linear correlation between these two methods (r=0.9988, P<0.05). Both OCT and histology showed that the skull thicknesses of older zebrafish were markedly higher than younger zebrafish (P<0.05), but no significant difference was found between zebrafish with the same age but different body lengths (P>0.05). This study demonstrated the age of zebrafish, not their body length can represent their developmental stages.OCT providing a powerful tool for noninvasive and nondestructive procedures in analyzing bone growth processes in long-time studies.