To develop a packaging film for 'Maehyang' strawberries, an edible film containing antimicrobial grapefruit seed extract (GSE) was manufactured. Addition of GSE to the rapeseed protein-gelatin (RG) film inhibited the growth of pathogenic bacteria such as Escherichia coli O157:H7 and Listeria monocytogenes. Packaging of 'Maehyang' strawberries with the RG film containing 1.0% GSE decreased the populations of total aerobic bacteria and of yeast and moulds in the strawberries by 1.03 and 1.34 log CFU g )1 , respectively, after 14 days of storage, compared to that of the control. Sensory evaluation of the GSE-RG film-packaged strawberries produced better sensory scores than did the control. These results suggest that RG film containing GSE can be used to package strawberries and to extend shelf life.

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Summary Barley protein (BP) was extracted from barley flour, and a BP film was prepared. To improve the physical properties of the BP film, nano‐clay was incorporated. Among the films prepared, the composite film containing 4% BP/1% Cloisite Na+ had the best physical property. The composite film containing grapefruit seed extract (GSE) was prepared and used as a packaging film for the button mushroom. Packing of the mushroom with this film inhibited microbial growth during storage. After 7 days of storage, packaging of mushrooms with the film containing 0.7% GSE reduced the populations of total aerobic bacteria and yeast and moulds by 0.95 and 0.58 log CFU/g, respectively, compared with the control. These results suggest that the BP/Cloisite Na+ film containing GSE can be used as an environment‐friendly packaging for maintaining mushroom quality during storage.

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