SUMMARYA total of 17 isolates of Vibrio mimicus from patients, 29 from environment and 2 from food was examined for toxigenicity. Sixteen (94 %) clinical isolates and one (50%) from food produced TDH-like toxin, whereas none of the environmental isolates did so. The food from which V. mimicus with TDH-like toxin production was isolated, was one which had caused food poisoning. Only one environmental strain produced CT-like toxin, whilst ST-like toxin was not detected from any strains tested.

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Vero toxin-producing E. coli O111:H-was isolated from 2 cases of patients with severe abdominal pain and bloody diarrhea and were diagnosed as appendicitis. E. coli from both cases produced both VT1 and VT2.

The effect of steam on sandpit disinfection was investigated by supplying steam into sandpits covered with a sheet cover. To evaluate the effect, total number of viable bacteria, number of aerobic sporeforming bacteria, coliforms and fecal coliforms were measured four weeks before, just before, immediately after, two and four weeks after the disinfection. Disinfection of coliforms and fecal coliforms was accomplished when temperature of sand reached more than 60 degrees C. Total number of viable bacteria and number of aerobic sporeforming bacteria also reduced by disinfection with steam. However, after 2 and 4 weeks of disinfection, number of coliforms and fecal coliforms recovered to the same level before disinfection, and total viable count also restored its number. In addition, a significant increase in the number of aerobic sporeforming bacteria was noted both after 2 and 4 weeks of disinfection. These results indicate that the effect of steam disinfection in transient and can not be used for the routine method of disinfection in sandpit.