E4BP4, a basic leucine zipper transcription factor, contains a DNA-binding domain closely related to DBP, HLF, and TEF, which are PAR proteins. Here, we show that the phase of e4bp4 mRNA rhythm is opposite to that of the dbp, hlf, and tef rhythms in the suprachiasmatic nucleus (SCN), the mammalian circadian center, and the liver. The protein levels of E4BP4 and DBP also fluctuate in almost the opposite phase. Moreover, all PAR proteins activate, whereas E4BP4 suppresses, the transcriptional activity of the reporter gene containing a common binding sequence in transcriptional assays in vitro. An electrophoretic mobility shift assay demonstrated that E4BP4 is not able to dimerize with the PAR proteins, but is able to compete for the same binding sites with them. Furthermore, we showed sustained low e4bp4 and high dbp mRNA levels in mCry-deficient mice. These results indicate that the E4BP4 and PAR proteins are paired components of a reciprocating mechanism wherein E4BP4 suppresses the transcription of target genes during the time of day when E4BP4 is abundant, and the PAR proteins activate them at another time of day. E4BP4 and the PAR proteins may switch back and forth between the on-off conditions of the target genes.

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Contrary to widespread incise CAD/CAM, little is known about the dental application of 3D printing, especially the possibility of using 3D printing with organic material. The resin patterns of a full crown model were created by four types of 3D printers, a thermofusion device (CX), a digital light processing stereo-lithograph device (B9), a laser stereo-lithograph device (DW) and a multi-jet modeling device (PJ). The dimensional accuracy of the outer and inner diameters and depths of the created model crowns, and the surface roughness of sidewall were investigated. The outer diameters were smaller than the designed value in most conditions. The inner diameters were smaller than the designed value in any enlargement ratio except B9. The depth of CX or B9 tended to become shallower, though that of PJ or DW tended to increase. The surface roughness along the tooth axis direction was greater than that perpendicular to the tooth axis.