One new flavanone, 4'-hydroxyisorottlerin (2), and two new chalcone derivatives, kamalachalcones C (3) and D (4), were isolated from Mallotus philippensis (kamala tree). The largest compound (4; M r 1098 g/mol) was shown to possess a unique, fused-ring system made of two hydroxy-chalcone units, giving rise to eight fused benzene/pyran units. From the same plant, the following six known compounds were also isolated: kamalachalcone A (5) and B (6), isoallorottlerin (7), isorottlerin (8), 5,7-dihydroxy-8-methyl-6-prenylflavanone (9); 6,6-dimethylpyrano(2'',3'': 7,6)-5-hydroxy-8-methylflavanone (10), and rottlerin (1). The structures of the new compounds were confirmed by in-depth spectral analyses, including 2D-NMR techniques, and the full 13 C-NMR assignments of the known flavanones 1 and 7 ± 10 are published for the first time.
The type and eight strains of Flavobacterium indologenes were clearly differentiated from the type and two reference strains of Flavobacterium gleam by deoxy-
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