As the basic research for the study of mechanism of ventilation in submerged fer mentations, the formula of carbon dioxide in the liquid phase was investigated. Carbon dioxide in liquid phase was mainly classified into two groups. One was a gaseous form and the other was a dissociated form. Carbon dioxide was considered to be a weak acids when it dissociated into ionic form, and the dissociation of carbon dioxide was expressed in Henderson-Hasselbalch's equation. The results in this study showed that those rules concerning the dissolution and dissociation of carbon dioxide held for a model liquid system.
Penicillin-resistant mutants were derived from Corynebacterium hydrocarboclastus R-7. One of them produced 84g/liter of L-glutamic acid from hydrocarbon, though its parent strain •õ This paper was presented at the 19th Symposium on Amino Acid and Nucleic Acid held on 1st Decem ber,
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