IntroductionCyclamen (Cyclamen persicum Miller) is a popular plant for horticulture, and for many years, sales of seeds and saplings have benefited the economy of the Ena-Nakatsugawa district, Gifu Prefecture, Japan. This success can be attributed, in part, to the close attention that gardeners pay to diseases affecting the production of seeds and saplings. Several diseases of cyclamen are caused by fungi and bacteria, such as anthracnose (Colletotrichum gloeosporioides), bacterial bud blight (Pseudomonas marginalis pv. marginalis), fusarium wilt (Fusarium oxysporum Schlechtendahl f. sp. cyclaminis), gray mold (Botrytis cinerea), bacterial soft rot (Erwinia carotovora subsp. carotovora), and bacterial leaf blight (Pantoea agglomerans) (Database of Plant Diseases in Japan, NIAS GenBank, https://www.gene.affrc.go.jp/databases-micro_pl_dis-eases_en.php). In particular, anthracnose and bacterial leaf blight frequently occur in Gifu.Among several bacterial species with fungicidal activity, Bacillus subtilis is well characterized (Weller, 1988). Most fungicidal B. subtilis secrete iturin group compounds, which consist of linear alkyl fatty acids and circular depsipeptides, including D-amino acids, as a highly active fungicidal component (Maget-Dana

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