Abstract·The C-banded karyotype of Henrardia persica (2n=2x=14), an annual self-pollinated grass species was studied in two natural populations using aceto-iron-hematoxilin staining and C-banding technique. It was revealed that the karyotype of this species is asymmetric and consists of 4 pairs of sub-acrocentric and 3 pairs of acrocentric chromosomes. One of chromosomes has a satellite located on the end of its long arm. Each chromosome having a unique C-banding pattern is easily recognizable.