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A glassy carbon electrode (GCE) modified with electropun tricobalt tetroxide nanoparticles decorated carbon nanofibers (Co 3 O 4 -CNF) were first applied for the determination of L-tryptophan (L-Trp). The synthesized Co 3 O 4 -CNF were characterized by scanning electron microscopy, Raman, X-ray diffraction and electrochemical impedance spectroscopy. The electrochemical detection of L-try was successfully conducted in 0.1 M phosphate solution (pH 2). The linear relationship of L-Trp was in the range of 0.005-40 μM and the detection limit reached 0.002 μM (S/N=3) with little interference from other amino acids.

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