Background: DGAT2 catalyzes the final and committed step of triacylglycerol (TG) biosynthesis. Results: Co-immunoprecipitation experiments and in situ proximity ligation assays showed that DGAT2 and MGAT2 interact. Conclusion: DGAT2 can utilize diacylglycerol generated by MGAT2 for TG synthesis. Significance: These findings provide insight into the spatial arrangement of enzymes involved in triacylglycerol biosynthesis.

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In order to solve the Distance-constrained Capacitated Vehicle Routing Problem ( Distanceconstrained CVRP ), the cellular genetic algorithm(CGA) is used in this paper. A new crossover operator--SAX which can better reflect the self-adaptability of CGA is proposed, and three types of neighborhood are introduced to analyse the search performance of CGA in Distanceconstrained CVRP . Two instances are introduced to show the feasibility of CGA in solving Distance-constrained CVRP . The experimental results show that the performance of CGA is obviously better than that of the traditional genetic algorithm, and the optimization results of the vehicle routings are improved. Especially, the Moore neighbor structure indicates better search efficiency for SAX crossover operator. Because of the strong searching ability, CGA can effectively solve the optimization of vehicle routing problems. ISCC2017