We studied cell cultures isolated from the pulp of third molar germ of an adult human and from the skin of a human fetus on gestation day 10. Both cultures expressed similar repertoire of surface markers typical of multipotent mesenchymal cells (CD44, CD90, and CD105). Under in vitro conditions, dental pulp cells were more susceptible to factors inducing their differentiation into adipogenic, chondrogenic, and osteogenic lineage cells.

The properties of reaction-associated calcium-phosphate materials obtained by pressing paste based on a-tricalcium phosphate with subsequent holding in different solutions are presented. The phase composition, solubility and mechanical characteristics of the materials obtained are studied and biological tests in vitro are performed.

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Reaction of norbornadiene (1) with arenesulfenyl chlorides occurs nonselectively and leads to a mixture of isomeric β-chlorosulfides and rearrangement products [1,2].We have established that the only direction for the reactions of diene 1 with 3-methoxycarbonyl-2-pyridinesulfenyl chloride (2) and 4,6-dimethyl-2-pyrimidinesulfenyl chloride (3) in nitromethane in the presence of lithium perchlorate at 20°C is stereospecific cycloaddition of the sulfenylating reagent at the multiple bond, with closure of the ring by the nitrogen atom of the thiohetaryl moiety and formation of condensed systems 4, 5 in 87% and 75% yields respectively. S N MeOOC S N N Me Me N SCl COOMe N N Me Me SCl + ClO 4 _ 4 + ClO 4 _ 5 3 2 MeNO 2 -LiClO 4 1 -LiClIn the 1 H NMR spectra of compounds 4,5, the signals from the protons of the CHS and CHN + moieties appear as doublets with spin-spin coupling constant 7.9-8.0 Hz, which suggests an exo-cis configuration for these products [3,4].The 1 H and 13 C NMR spectra were taken on a Bruker DRX-500 (500 MHz and 125 MHz respectively) in DMSO-d 6 .Sulfenylation of Diene 1 (General Procedure). A solution of LiClO 4 (1.06 g, 10 mmol) in nitromethane (30 ml) and a solution of sulfenyl chloride 2 or 3 (10 mmol) in nitromethane (10 ml) were added to a solution of compound 1 (0.92 g, 10 mmol) in nitromethane (20 ml) at 20°C. After 10 min, the LiCl __________________________________________________________________________________________

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