Aim: To evaluate microviscosity and sorption capacity of erythrocyte membranes (SCEM) from patients with laryngeal cancer (LC). Materials and Methods: Samples from 35 patients with LC of stages II and III and 20 healthy volunteers were investigated by electron paramagnetic resonance with Bis(1-oxyl-2,2,6,6-tetramethylpiperidinyl-4)-ester of 5,7-dimethyladamantane-1,3-dicarbonic acid (AdTEMPO) probe. SCEM was evaluated by amount of unabsorbed methylene blue. Results: Microviscosity of erythrocyte membranes was determined by the effective rotational diffusion correlation times (τeff) and a decrease in radical spectrum signal intensity per hour. The most apparent decrease in mobility of the AdTEMPO in erythrocytes was observed prior to washing of erythrocytes with 0.9% NaCl for 5 min after probe insertion. The deceleration after 60 min was observed only in stage II LC. τeff was at control values after washing of erythrocytes of stage II LC 5 min after probe insertion and was significantly reduced in stage III LC in comparison to control. Radical spectrum signal intensity per hour in samples of stage II and III patients prior to and after washing of erythrocytes was on average 1.5-fold higher than that of control. SCEM in samples of stage II and III LC was found in 40 and 33% cases, respectively and was on average significantly reduced in comparison to control. Conclusions: The initial interaction of AdTEMPO with erythrocyte membranes of stage II and III LC patients is accompanied by an increase in τeff, indicating deceleration of probe rotation. τeff of the probe in membranes remains unchanged in 60 min, indicating changes in the structural organization of lipid bilayer and its associated proteins in particular. The similarity of SCEM for both studied groups reflects the pathological changes in function of erythrocyte membranes.

Introduction. Cellular membrane barrier alterations lead to metabolic and functional disorders. However, in the case of laryngeal cancer (LC) they are insufficiently studied. The aim of the study – to learn the nature of the interaction of erythrocyte membranes with introduced spin probes as an indicator of changes in the barrier function of membranes at LC. Research Methods. Samples of the erythrocyte membranes from 40 patients with LC stages II and III and 20 healthy volunteers were probed by EPR with AdTEMPO test. Microviscosity of erythrocyte membranes was determined by the τeff and the decreasing in RSSI. The content of MWM was identified in the blood plasma and in erythrocyte. The partition coefficient between blood plasma proteins and erythrocyte glycocalyx was calculated. SCEM was evaluated by amount of unabsorbed methylene blue. Results and Discussion. It was established that LC patient’s endogenous intoxication is characterized by excessive accumulation of the total pool of MWM both in blood plasma and glycocalyx of erythrocyte. SCEM was significantly decreased in samples of both LC stages in comparison to control. The most apparent decline in τeff was observed prior to washing of erythrocytes for 5 min after probe insertion. The deceleration after 60 min was observed only in LC stage II. The value of τeff was at control values levels after washing of erythrocytes of LC stage II 5 min after probe insertion and was significantly reduced in stage III LC in comparison to control. RSSI in samples both stage of patients prior to and after washing of erythrocytes was on average 1.5-fold higher than that of control. Conclusions. It was established that the LC patient’s endogenous intoxication is characterized by excessive accumulation of the total pool of MWM both in blood plasma and glycocalyx of erythrocytes, activation of catabolic processes in plasma, redistribution of MWM between the pool of erythrocyte proteins, which corresponds to the second stage of endotoxicosis. The reduction of the SCEM is shown, which is a manifestation of pathological changes in the surface functional activity of erythrocyte membranes. The effectiveness of AdTEMPO for the eva­luation of microviscosity of erythrocyte membranes in patients with LC was confirmed.

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A comparative study of the induced platelet aggregation in patients with laryngeal cancer to determine the most revealing informative violations. It was compared evaluation of results of platelet aggregation in the blood plasma of patients with laryngeal cancer compared to the healthy persons. It was found intensification of ADP induced platelet aggregation in the concentration range which was used. At the same time with the increase the number of patients with platelet hyperaggregation reliable platelet decrease in blood and increase of patients with thrombocytopenia at the III-rd stage of laryngeal cancer are observed. In patients with lI-nd and III-rd stage of laryngeal cancer was found increase in the level, rate and aggregation time compared to the healthy persons. The most significant violations observed in the II-nd stage of the cancer process.

It was compared evaluation of results of erythrocytes aggregation parameters in patients with laryngeal cancer compared to the healthy persons. It was found that in patients with laryngeal cancer II-d and III-d stage of the tumor process against the background of increasing the degree of aggregation and the time relative to the corresponding reference data, there is a simultaneous decrease in its speed of aggregation. In addition, the study has shown increase in average weight molecules and fibrinogen levels in the blood plasma of patients with laryngeal cancer, to a lesser extent – with the II-d stage of the disease, and their more significant violations were observed in the III-d stage of the cancer process. Sialic acid content was increased at II and III-d stages of cancer of the larynx in the same degree compared to the healthy persons. It was established opposite changes of basic parameters of erythrocytes aggregation. The most significant violations of aggregation degree were observed in the II-d stage of the cancer process. Growing of levels of the average weight molecules, fibrinogen and sialic acid in the blood plasma of patients with laryngeal cancer indicates the presence of metabolic intoxication and the acute phase of inflammation, the severity of which to a certain extent depends on the stage of the disease.