3-D quantification of biomolecular covers using surface plasmonpolariton resonance experiment Chegel, V.; Chegel, Yu.; Guiver, Michael; Lopatynskyi, A.; Lopatynska, O.; Lozovski, V. Sensors and Actuators B 134 (2008) The concentration of surface molecules N s and components of molecular susceptibility jl (ω) can both be determined from surface plasmon-polariton resonance (SPPR) experiments, instead of effective layer thickness and index of refraction, which are usually determined. The theoretical consideration of a molecular layer as monolayer of separated 3D-oscillators provides a new perspective for investigating molecules during SPPR experiment. It is shown that SPPR response and the form of the reflective curve depend on the form of a biomolecule and its orientation relative to the surface of the metal-carrier of plasmon oscillations. The experimental data for immunological reaction for the calculation of surface molecular concentration and mass of biomolecular covering are presented.