N-Substituted isatin derivatives were prepared from the reaction of isatin and various bromides via two steps. Bioactivity assay results (in vitro tests) demonstrated that some of these compounds are potent and selective inhibitors against SARS coronavirus 3CL protease with IC50 values ranging from 0.95 to 17.50 microM. Additionally, isatin 4o exhibited more potent inhibition for SARS coronavirus protease than for other proteases including papain, chymotrypsin, and trypsin.

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Severe cardiac adverse effects are often related to intravenous phenytoin overdose. However, there is no reported cardiotoxicity resulting from oral overdose of phenytoin. We report a patient with post-traumatic epilepsy who received oral phenytoin for five months and developed life-threatening junctional bradycardia, with his serum phenytoin level reaching up to 91 microg/mL. The patient was successfully treated with temporary transvenous pacemaker implantation for his severe bradycardia and hypotension. To our knowledge, our patient had the most serious cardiovascular toxicity ever reported with chronic oral phenytoin overdose. From emergency department (ED) physician's perspective, when a patient with dysrhythmias and cardiovascular collapse is presented to the ED, severe phenytoin overdose should be considered in patients on oral phenytoin with hyperbilirubinemia, hypoalbuminemia, and severe electrolyte imbalance.

Future generations of wireless networks will place great demands on the performance of radio access technology and the real-time multimedia applications provided by these new wireless environments. During the mobility between heterogeneous wireless networks, the quality of multimedia services for users depends heavily on seamless handover which should be provided by the middleware of IP to MAC protocol stack. IEEE 802.21 is designed for this reason which provided the basic platform for seamless handover between heterogeneous wireless networks. In this paper, a dynamically adjusted buffer component with algorithm for prediction of the buffer size is proposed to enhance the functionality of IEEE 802.21. The mechanism of proposed scheme included Service Specific Layer which decides the opportunity of handover, and adjusted Buffer which stores the temporal streaming data for applications between handover. The services and communication flows among service specific layer, buffer and IEEE 802.21 Media Independent Handover function are described and analyzed. According to analysis of the connection process and simulation, buffer prediction and pre-allocation could indeed reduce the influence of handover between heterogeneous wireless networks.Keywords-Heterogeneous network handover, seamless handover, Media Independent Handover, IEEE 802.21