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The effecT of magneTophoToTherapY on morphological changes of Tissues of paThologicallY changeD perioDonTium rubnikovich s. p. 1 , maizet a. i. 1 , Denisova Yu. l. 2 , Bykova n. i. 3 , arutyunov a. V. 3 , Kopylova i. a. 4 , avanesyan r. a. 4 1 Belarusian medical academy of postgraduate education, minsk, republic of Belarus 2 Belarusian state medical university, minsk, republic of Belarus 3 Kuban state medical university, Krasnodar, russian federation 4 stavropol state medical university, russian federation

The aim of the study is to develop and scientifically justify a new method for treating patients with gingival recession using autologous mesenchymal stem cells of adipose tissue. Objects and methods. A complex treatment was conducted in 93 patients with an anatomical recession of the gums in combination with dentoalveolar anomalies at the age of 20 to 34 years. Patients were divided into 3 groups (1st, 2nd and 3rd groups) depending on the treatment used. All patients underwent periodontological treatment. In the second group, patients included periodontological treatment including orthodontic equipment, in the third group, orthodontic treatment and the use of a cell transplant on the basis of a mixture of autologous mesenchymal stem cells and sterile bioplastic collagen material “Collost”. Results and discussion. A comprehensive assessment of the periodontal tissue of the three groups showed that among the patients who received the standard treatment complex, a good condition of the periodontal tissues 1 to 2 years after treatment was noted only in 16.7% of the patients of the 1st group. Inclusion of orthodontic treatment made it possible to obtain good results in 25% of patients in the 2nd group, which is 8% more than in the 1st group. At the same time, good results of treatment were revealed in 94% of patients in group 3, who included periodontological and orthodontic treatment using autologous mesenchymal stem cells, which confirms the need for measures aimed at normalization position of teeth, dentition and bite with the use of small orthodontic long-acting forces, improving microcirculation of periodontal tissues and restoring the contour of the marginal gum. Conclusions. Inclusion of autologous mesenchymal stem cells in the complex treatment of patients with gingival recession is accompanied by a 1.6-fold decrease in the prevalence and gum recession (1.15 mm), and tissue volume increased 2.6-fold, stimulation of periodontal microcirculation processes (in terms of capillary the periodontal pressure was 1.1 times and the intensity of periodontal microcirculation was 3.1 times), as well as the normalization of the position of teeth, dentition and occlusion, which allowed to obtain good long-term results in 94% of patients.

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The aim of the study was to assess experimentally the possibility of using mesenchymal stem cells (MSC) and bioplastic material Collost for gingival recession treatment based on the data of morphological alterations in the tissues of pathologically transformed periodontium. Materials and Methods. Random-bred white female rats (60 specimens) were used as a model in the experiment. The operative intervention was performed in the area of incisors of the upper and lower rat jaws. Before the investigations, 1-2 ml of the adipose tissue were harvested from one specimen under sterile conditions in order to obtain allogeneic mesenchymal stem cells. All animals were divided into 5 groups (10 rats in each group) depending on the selected method of treatment: group 1-experimental gingival recession without treatment; group 2-treatment with physiological solution; group 3-experimental gingival recession treatment with 7% Collost gel; group 4-experimental gingival recession treatment with MSC; group 5-experimental gingival recession treatment with MSC and 7% Collost gel. The control group, intact rats, consisted of 10 laboratory animals with a healthy gingiva. The creation of a model of the experimental gingival recession was carried out by mechanical V-shaped excision of periodontal tissues. Results. The isolated form of 7% Collost gel (a bioplastic collagen material) serves in the process of biodegradation as a matrix for the formation of the native connective tissue, provides adhesion of the stem cells and their transformation into pro-and fibroblasts. 7% Collost gel facilitates the reduction of gingival recession and gingival pocket depth, stimulates the process of epithelization. Injections of cell transplant suspension in physiological solution activate the processes of fibroblast differon cell proliferation and cell transformation. The cell transplant suspension in the sterile bioplastic 7% Collost gel enhances the gel and stem cell effects, contributes to the elimination of the atrophic and dystrophic changes in the gums, increase of mechanical strength, reduction of gingival recession and gingival pocket depth.