To study spreading profile, cytoskeleton structure organization, and proliferation of bone marrow stromal cells in the course of cultivation on the glass-crystalline material (GCM) surfaces, with different chemical composition and solubility. Methods. GCMs with different CaO: P 2 O 5 ratios were used. The actins cytoskeleton in cells was visualized using fluorescent TRITC-conjugated phalloidin. Cell proliferation was studied using MTT test. Results. The cell cultivation on highly soluble B series GCM (Ca/P = 5 superficial ratio) led to appearance of fibroblast-like cells, the actin cytoskeleton filaments of which were uniformly distributed within cytoplasm. In this case, proliferation dynamics was similar to that] under cultivation on plastic. Ca/P ratio reduction on the surfaces of A and C series of GCMs to 1.4 ÷ 2.58 resulted in a decrease of spreading area and proliferation index (up to 2-3 times) relative to the control. Conclusions. Main factors, determining the cells interaction behavior with GCM are the materials solubility and Ca/P superficial ratio.
K e y w o r d s: glass-crystalline materials, bone marrow stromal cells.New possibilities for injured organs and tissues recovery are provided with one of the most upto date interdisciplinary area of medicine, namely tissue engineering. It is based on the synthesis of new bioactive materials, with a range of chemical properties that aimed to enhance the treatment efficiency, enhance regeneration or substitute damaged tissues and organs [1]. Bioreactive calcium phosphate scaffolds with cer-tain CaO/P 2 O 5 ratio such as glass, ceramics and glass-ceramic materials (GCM) are widely used for arthroplasty nowadays. GCMs based on crystalline calcium phosphate (CP) are the most promising for the orthopedic and dental implants creation [2,3,4].GCMs are much more durable and fractionresistant in comparison to hydroxyapatite ceramics, which is determined by their crypto-
Molecular and Cell Biotechnologies
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