The article presents an investigation of the influence of the corona discharge with needle-ring electrode configuration on the efficiency of Aspergillus niger spore germination and Cyathus olla mycelium development. Current-voltage characteristics were measured for different distances between the needle and the metal ring. The plot of the dependence of conductance on voltage was built and the corona discharge breakthrough voltage was determined to be (2.7±0.1) kV. The treatment of Aspergillus niger spores was conducted at different development stages: at the germination stage (corona discharge treatment 1 day after the seeding) and dormant state (corona discharge treatment on the day of seeding). The growth rate of Cyathus olla mycelium in samples treated by corona discharge and in control group samples was investigated. The influence of the corona discharge on the potato dextrose agar growth medium before the seeding of Cyathus olla was studied. The pure cultures were obtained from the Culture Collection of Fungi at Kyiv University (FCKU) at the «Institute of Biology and Medicine» Educational and Scientific Centre of the Taras Shevchenko National University of Kyiv.