Background:The catalytic cycle of Top2 has multiple steps of reactions with corresponding conformational changes. Results: We can monitor conformational dynamics of hsTop2␣ upon binding with cofactors and the inhibitor ICRF-193. Conclusion: Coordinated movements of the N-gate and DNA gate of hsTop2␣ occur with binding to cofactors and an inhibitor. Significance: Structural dynamics of hsTop2␣ can be detected during key catalytic steps.
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