Based on the results of immunological assessments and rejection of the material by phenotype, 327 sources and 79 donors of immunity were selected from spring wheat, 27 and 23 from winter wheat, respectively. Applying PCR diagnostic method, Lr genes were found in the selected samples of spring wheat. Screening revealed the presence of the following genes in the studied samples. They are Lr9, Lr19, Lr24, Lr 34, Lr1, Lr10, Lr20 and Lr26. Within selection samples of the Central Russian branch, the prevalence of lines with the Lr19 gene in combination with inefficient genes Lr10, Lr20 and Lr26 was revealed. Spring soft wheat cultivation lines adapted to zonal conditions have been developed, combining in their phenotype resistance to the main phytopathogens of the region with valuable economic properties. In 2019, a variety of spring soft wheat in memory of Plahotnik was transferred to the State Export Commission for testing.
For the period of 2020, the species composition of winter wheat leaf blotch pathogens on the territory of the Central Chernozem region was studied. It is shown that the Zymoseptoria tritici species dominates in the pathogenic complex during all phases of wheat vegetation. Under the conditions of the year, the other two species - the pathogen of the disease: Parastagonospora nodorum and Parastagonospora avenae f. sp. triticea had no economic significance. The populations of the species Zymoseptoria tritici, formed on winter and spring wheat cultivats according to morphological, physiological and pathogenic properties, were studied. It is shown that black corrugated colonies with an average growth rate and high sporulation predominate. Using monogenic lines, virulence phenotypes were determined in isolates taken from infectious wheat material. Seven virulence phenotypes were identified. All isolates hit the test lines from 2 to 4 points, therefore they were characterized by wide virulence and high aggressiveness. The information obtained is necessary for the development of a methodology for resistance breeding.
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