Objective: To evaluate the potency of the fraction of marine sponge Stylissa carteri in inducing cell death, inhibiting spheroid growth, and its impact on pro-apoptotic protein Mcl-1S in breast cancer cells. Methods: Stylissa carteri were collected from Pramuka Island followed by ethanol extraction and ethyl acetate fractionation. To evaluate the cytotoxic effect of fraction, the HCC-1954, MDA MB 231, and MCF-7 cells were treated with the fraction of Stylissa carteri and MTT assay was then performed. The effect on spheroid growth was evaluated in HCC-1954 cells. The combined effect of the ethyl acetate fraction and paclitaxel were analyzed using combination index (CI) and immunoblotting on the pro-apoptotic protein Mcl-1S. Furthermore, compounds in this fraction were identified using GC-MS. Results: Data showed that both the MDA MB 231 and HCC-1954 cells were interestingly more sensitive to the fraction as compared with MCF-7 cells. The IC 50 of the ethyl acetate fraction on HCC-1954, MDA MB 231 and MCF-7 were 4.1 µg/ml, 3.9 µg/ml, and 123.8 µg/ml, respectively. In addition, the fraction triggered spheroid destruction within 10 days. The CI of paclitaxel and ethyl acetate fraction of Stylissa carteri were less than 0.52. Moreover, this combination induced upregulation of the Mcl-1S protein. Furthermore, some fatty acid-based structures were predicted as the major compounds in this fraction. Conclusion: The ethyl acetate fraction of Stylissa carteri induces cell death and spheroid destruction in aggressive breast cancer cells. It has a synergistic cytotoxic effect with paclitaxel on MDA MB 231 cell death and upregulates Mcl-1S protein.

PLWHA (People with HIV AIDS) is not just having problems physically or biologically, but also experience psychological problems, social and spiritual. When a person has been diagnosed with the HIV virus in the psychosocial assessment of PLWHA will withdraw from the environment. Disruption of self esteem affect communication PLWHA who are part of the process of social interaction. The purpose of this research is to know the extent of fatherly pride relationship with social interactions in people with HIV-AIDS. The research was conducted at Yayasan Lantera Minangkabau Support Padang from April to May 2013. This research is correlational cross-sectional approach. Total sample in this study of 45 PLWHA using total sampling technique. Collecting data using a questionnaire using a likert scale for the variable self-esteem and social interaction. Univariate data analysis done for the bivariate frequency distribution and performed with Spearman's test. The results showed as much as 91.4% of PLWHA have positive self esteem and 64.4% of PLWHA with good social interaction. There was no significant association between self-esteem and social interaction. The result is expected to be information and increase knowledge in promoting nursing care for PLWHA.