p210bcr-abl-Related tyrosine kinase activity has been shown to cause chronic myelogenous leukemia (CML), a disease of bone marrow stem cells. Having previously demonstrated that the aptameric oligonucleotide, ODN-1, could inhibit p210bcr-abl kinase activity, the current study sought to determine if ODN-1 could selectively inhibit the growth of CML cells relative to that of normal bone marrow. ODN-1, when introduced by electroporation into peripheral blood mononuclear cells (PBMC) from patients with CML, decreased the number of committed progenitors (CML CFU-GM) by an average of 67%+/-19% (mean+/-SEM, range 28-98%). Treatment of CML PBMC with ODN-1 was also shown to decrease the number of more primitive cobblestone area-forming cells (CAFC) by 35%-87%. In contrast, there was little suppressive effect by the combination of electroporation and ODN-1 on either CFU-GM or CAFC numbers from normal donor bone marrow. These studies suggest that inhibition of p210bcr-abl protein-tyrosine kinase (PTK) activity by ODN-1 is associated with some degree of selective growth inhibition of p210bcr-abl-transformed cells. p210bcr-abl kinase inhibitory agents may be useful for the ex vivo purging of bone marrow or peripheral blood progenitor/stem cells in the setting of autologous transplantation for CML.

Ship navigation performance more and more attention in real sea conditions.To study the properties of ship motion in waves, Applling hydrodynamics calculation software for AQWA-line and AQWA-AGS modules and taking into account the role of random wave load, 2450DWT a small oil tanker in simulation and analysis of a campaign response under sea conditions, Calculate shipping in several waves under the angle of vertical and horizontal swing, heave, pitch, roll and the first rolling response amplitude operators of RAOs, additional parameters such as quality and radiation damping. Application of spectrum analysis method, ITTC wave with two parameters, which strike the spectral density of ship motion, and finally using Excel to calculate sprawling forecast prediction of ship under irregular wave.

testing stand that is able to imitate the vibration of seeder field surface stirred up is designed to analyse the effect of vibration on the seed metering function of pneumatic seeder. The signal of vibration data is collected by tape recorder on the testing spot in testing process and then the vibration signal is analysed. A conclusion is reached based on the analysis of testing result that: the trait vibration of seeder is an approximate periodic function, which may be approximately simulated with sine exciting signal; the trait vibration frequency of seeder ranges form 0 to 68 Hz, the maximum acceleration of vibration makes 25m/s2, and the minimum excited vibration thrust of vibration exciter is 500N. A testing stand for seed metering is designed by taking these parameters as the criterion to simulate field surface excited vibration and analyse the seed metering dynamic characteristic of seed metering device.

Using the finite element ANSYS workbench platform Hydrodynamic Diffraction and Hydrodynamic Time Response Module, taking a self-elevating offshore mooring location as a study case for the corresponding value calculated the dynamic response of the platform. Studied in the wind, waves and currents under load, platforms and mooring ropes coupled motion in case of time-domain response analysis, and get mooring ropes mooring and platform displacement force-time curve. Methods discussed in this paper have a certain reference value for offshore platform mooring positioning.

This paper focus on the ship form optimization of a 62-feet yacht and uses experienced method based on amount of statistical data to change the width of knuckle lines in order to reduce the resistance of the hull, including comparison of performance of stability and see keeping.