An elegant GlaI–EXPAR strategy is proposed which allows accurate detection of site-specific DNA methylations with ultrahigh sensitivity and specificity.
A novel one-step microRNA assay is developed based on a target-triggered loop-mediated isothermal amplification (TT-LAMP) mechanism, which enables the accurate detection of as low as 100 aM (1 zmol) microRNA with simple one-step operation by using only one-type of DNA polymerase.
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