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Ovarian cancer has always entangled most women. Studies have shown that the prevalence of ovarian cancer ranks third in female reproductive malignancies, and the mortality rate has always been the highest. The reason is mainly because the diagnosis and treatment of preovarian cancer has always been a big problem. However, the emergence of laparoscopy can well solve this problem, especially laparoscopy assisted by blockchain technology, which plays a huge role in the overall staging of ovarian cancer. This article proposes the application research of laparoscopy in the comprehensive staging of ovarian cancer based on electronic medical blockchain technology. First of all, this article uses the literature method to study the clinical characteristics and surgical classification of ovarian cancer, as well as the application status of blockchain technology and laparoscopic technology. Secondly, it designed an application experiment based on electronic medical blockchain technology to assist laparoscopy in the comprehensive staging of ovarian cancer and analyzed the comparison of the laparoscopic group and the control group in the comprehensive staging of ovarian cancer. The results of the study showed that the amount of bleeding in the laparoscopic group was 103.5 ml, while the amount of bleeding in the control group was 141.1 ml; the proportion of tertiary pain in the laparoscopic group was 11.37%, and the proportion of tertiary pain in the control group was 31.82%. From this, it can be seen that, in the comprehensive staging operation for ovarian cancer, the laparoscopic group has less intraoperative blood loss than the control group and lower pain, and the treatment effect is better.

Background Two factors involved in regulation, long noncoding RNA Opa interacting protein 5‐antisense RNA 1 (lncRNA OIP5‐AS1) and microRNA‐147a, were found in cervical cancer. Therefore, the investigation of the specific regulation of miR‐147a by OIP5‐AS1 was performed in cervical cancer. Method The cervical cancer tissues were collected from patients with cervical cancer (n = 50). The expression of OIP5‐AS1, miR‐147a, proteins in epithelial‐mesenchymal transition (EMT) process and insulin‐like growth factor 1 receptor (IGF1R) were measured by quantitative real‐time polymerase chain reaction (qRT‐PCT) or western blotting. Cell motility and the relationship between OIP5‐AS1 and miR‐147a were detected or analyzed by wound healing test, Transwell assay, dual‐luciferase reporter assay, RNA binding protein immunoprecipitation assay or Pearson correlation in OIP5‐AS1, or miR‐147a over‐expressed and/or suppressed cervical cancer cells. Results OIP5‐AS1 showed the high‐expression and miR‐147a showed the low‐expression in tumor tissues collected from patients with cervical cancer and cell lines Hela, CaSki, Siha, and ME‐180. The low‐expression of OIP5‐AS1 suppressed the motility of Caski cells, as well as up‐regulated the level of E‐cadherin, which a key protein in EMT. There were targeting sites between miR‐147a and OIP5‐AS1. OIP5‐AS1 induced the down‐regulation of miR‐147a, so miR‐147a was inversely correlated with OIP5‐AS1. The down‐regulation of miR‐147a increased IGF1R and E‐cadherin, and these increases were alleviated by OIP5‐AS1 knockdown. Conclusion LncRNA OIP5‐AS1 promotes the migration, invasion and EMT of cervical cancer cells via targeting miR‐147a/IGF1R pathway.

Abstract. Objective To evaluate the effect of hysterectomy for reserving the uterine blood supply on ovarian endocrine function and on symptoms of menopausal transition. Methods Uterine benign lesions should be line the uterus times total resection in 100 patients were randomly divided into hysterectomy group of retaining uterus vascular supply group(research group,n=50) and traditional total hysterectomy group (the control group, n=50), comparing two groups in operation time, intraoperative bleeding ,postoperative fever and residual polyp, blood tests were taken to check the serum sex hormone levels change and clinical observation for perimenopausal symptoms before and postoperative three months, six months, one year and two years at the same time respectively. Results There was no significant difference between two groups in operation time, intraoperative blood loss, postoperative fever and residual polyp (P>0.05). There was no significant difference among research group before and after operation in serum sex hormones(P>0.05),the symptoms of the menopausal transition hardly appear; postoperative FSH, LH increased significantly in control group (P<0.05),E2 decrease (P<0.05), perimenopausal symptoms appeared more often. Conclusion The effect of uterus hysterectomy for retaining vascular supply on ovarian endocrine function is less than the traditional total hysterectomy, this operation has a certain importance to preserve ovarian function and delay the occurrence of premature ovarian aging.

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