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Plastic deformation behavior of bismuth single crystals have been investigated by tensile tests in principal axis at 298K and 423K, and influence of temperature on the plastic deformation behavior has been discussed. A combined deformation mechanism of twinning and slip was observed at 298K in most of axes tested, and elongation was given by mainly the slip inside the twin crystals. On the other hand, the slip deformation was the predominant mechanism for any axes at 423K and the twinning was not observed. {11 ̅ 02}<11 ̅ 01 ̅ > slip system was operated that was not activated at 298K, since the covalent bonds including in the slip system was depressed at 423K. Thus, the plastic deformation of bismuth changes to metal like behaviour at elevated temperature and anisotropy in the plastic deformation reduces.