Phosphonylation of inosine (Ino) has been achieved using inorganic diphosphonate (DP) in aqueous solution. The optimum condition for the phosphonylation of Ino with DP is Ino : DP = 1 : 10, pH 11 and 10 ºC. 2'-Phosphonylinosine and 3'-phosphonylinosine were synthesized with the total yield of more than 95 %. The phosphonylated products of Ino were stable at 10 ºC and pH 9. The reaction mechanism of Ino with DP was discussed.
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