BACKGROUNDCancer cells have a relatively high telomerase activity compared to normal cells, so that cancer cells have the ability for continued proliferation and uncontrolled mitosis. Telomerase is an enzyme responsible for the length of telomeres, DNA segments located at the ends of eukaryotic chromosomes. Natural materials such as rodent tuber (Typhonium flagelliforme) have anticancer potential. The purpose of the present study was to determine the effects of Typhonium flagelliforme extract on telomerase expression in HeLa cervical cancer and T47D breast cancer cells.

Cancer cells have a relatively high telomerase activity compared to normal cells, so the cancer cells have the ability to continue to proliferate and undergo mitosis uncontrolled. Telomerase is an enzyme which responsible for telomere length, a DNA segment that is at the end of eukaryotic cell chromosomes. Telomeres and telomerase play a role in the incidence of carcinogenesis. Natural materials such as taro mice (Typhonium flagelliforme) have potential as anticancer. The purpose of the study was to determine the effects of plant extracts of rat taro on the expression of telomerase in cancer cell Raji. The research method is experimental studies in some form cancerous cell culture cell line, Raji. Used as a control normal cells is Vero cell. The Culture medium used RPMI for Raji cell and M199 for Vero cell. The study consisted of three groups, control, doses of 1 ½ IC50 and IC50 doses. Expression of telomerase enzyme was measured by the Immunohystochemistry method (IHC). The results showed that the expression of telomerase in cancer cells showed values significantly higher than the normal cells (Vero). Giving mice taro plant extracts (Typhonium flagelliforme) were able to decrease the expression of telomerase significantly in both treatment doses. It was concluded that rodent tuber extract (Typhonium flagelliforme Lodd) can reduce the expression of telomerase in Raji cells, so that the rodent tuber extract (Typhonium flagelliforme Lodd) has potential as an anticancer through the expression of telomerase.

Telomeres are strands of non coding DNA at the ends of chromosomes that have the primary function to protect DNA from damage and maintain chromosomal stability. Physical exercise will increase the antioxidant activity can increase telomere proteins, lengthen telomeres and or protein networks associated with telomere so that the telomere remains long, or stopping telomere shortening. Telomere length was also associated with age. The purpose of the research was to determine telomere length of leukocyte cells in the KONI (Indonesian National Sports Committee) athletes in Jakarta. The research method is descriptive, by measuring telomere length using quantitative PCR on leukocyte cells. Samples are KONI athletes from several sports, including men and women athletes, with ages between 15-20 years. Used a control group (not athletes) is students of the Faculty of Medicine, University of YARSI. The results showed that there was no significant difference (p> 0.05) between telomere length group of athletes with the control group in both sexes. Similarly, telomere length between athlete male with female athletes also showed no significant difference (p> 0.05). It was concluded that physical exercise in athletes KONI at the age of 15-20 years had no effect on telomere length in leukocytes. The results of this study provide information about the telomere length in Indonesian athletes at an early age.

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Integrated Services Post (Posyandu) Cadres are a volunteer that helps to manage community health care issues. Normally, they play an important role in mother, child, and elderly health issue prevention and promotion. However, in the COVID-19 pandemic time, the role of Posyandu cadres should be optimized for the prevention of the disease. This research aimed to assess the efficacy of the utilization of WhatsApp in increasing knowledge of Posyandu cadres on preventing COVID-19, through Whatsapp group (WAG). The Quasi-experiment was conducted on 30 cadres in Bekasi Barat. They were invited to received counseling sessions using the Whatsapp Group (WAG) on how to prevent the disease and how to correctly use the mask. They were provided with all the counseling material that they could read before the question and ask session with the mentor. Changes in the knowledge level of the cadres were assessed using the pretest and post-test methods. There was a significant increase in the level of knowledge (Wilcoxon Signed Ranks Test, p = 0.004) which was indicated by a higher post-test score (mean 75.46) than the pre-test score (mean 60.69). The correct use of masks was the cadres’ most significant increase in knowledge post-WAG-counseling session. Most of the cadres enjoyed the education process through WAG and would like to use the educational materials as a tool to provide education to the wider community. Education through WAG is effective for increasing the knowledge of Posyandu cadres about COVID-19 prevention and can be emulated by cadres to educate the wider community