The onset of ventricular depolarization defines the start of the preejection period (PEP), which is commonly used as an index of myocardial contractility and sympathetic control of the heart. Although the fiducial point for this onset has traditionally been the onset of the Q wave of the electrocardiogram, other measurement points have also been used in the literature, including the peak of the Q wave (i.e., the onset of the R wave). Conceptual, physiological, and empirical considerations addressing the reliability and validity of these alternative metrics support the application of the Q-wave peak/R-wave onset as the fiducial point for PEP measures.

The root mean square successive difference (RMSSD) in heart period series is a time domain measure of heart period variability. The RMSSD is sensitive to high-frequency heart period fluctuations in the respiratory frequency range and has been used as an index of vagal cardiac control. By transfer function simulations, the RMSSD statistic is shown to represent a high-pass filter that effectively captures respiratory sinus arrhythmia but also passes lower frequency fluctuations that can include sympathetic influences. These simulations, together with analysis of actual heart period series, reveal that the RMSSD is biased by basal heart period. Although between-subjects levels of RMSSD covary highly with spectral estimates of high-frequency variability, within-subject RMSSD change scores account for only 50-60% of the variance in spectral estimates. The present findings raise caveats in the applications and interpretation of the RMSSD statistic.

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Terpinen-4-ol, a monoterpene component of the essential oils of several aromatic plants, exhibits antitumor effects. In this study, the antitumor effects of terpinen-4-ol and the cellular and molecular mechanisms responsible for it were evaluated and studied, respectively on human nonsmall cell lung cancer (NSCLC) cells. Our results indicated that terpinen-4-ol elicited a dose-dependent cytotoxic effect, as determined by MTT assay. Increased sub-G1 population and annexin-V binding, activation of caspases 9 and 3, cleavage of poly(ADPribose) polymerase (PARP), and a decrease of mitochondrial membrane potential (MMP) indicated involvement of the mitochondrial apoptotic pathway in terpinen-4-ol-treated A549 and CL1-0 cells. Elevation of the Bax/Bcl-2 ratio and a decrease in IAP family proteins XIAP and survivin were also observed following terpinen-4-ol treatment. Notably, terpinen-4-ol was able to increase p53 levels in A549 and CL1-0 cells. Diminution of p53 by RNA interference induced necrosis instead of apoptosis in A549 cells following terpinen-4-ol treatment, indicating that terpinen-4-ol-elicited apoptosis is p53-dependent. Moreover, intratumoral administration of terpinen-4-ol significantly suppressed the growth of s.c. A549 xenografts by inducing apoptosis, as confirmed by TUNEL assay. Collectively, these data provide insight into the molecular mechanisms underlying terpinen-4-ol-induced apoptosis in NSCLC cells, rendering this compound a potential anticancer drug for NSCLC.

In this paper we present a systematic study of the location of the convection reversal boundary for southward interplanetary magnetic field by using Defense Meteorological Satellite Program (DMSP) F13 and F15 spacecraft measurements during local summer seasons from 2000 to 2007 for both hemispheres. All the convection reversal boundaries are identified pass bypass by locating the highest‐latitude location where the plasma flow shows a large‐scale two‐cell pattern and reverses direction from sunward to antisunward. The location of the convection reversal boundaries are placed into 10 different categories based on By and the magnitude of southward Bz. Observations suggest that (1) the location of the boundary is well organized by the magnitude of Bz, being at lower latitudes for stronger negative Bz and also organized by the polarity of By, moving toward the dawnside/duskside when By changes from negative to positive in the northern/southern hemisphere; (2) the average latitudinal movement of the boundary associated with By changes is comparable to the average movement of the boundary with Bz changes; (3) an initial reconfiguration of the boundary near local noon is redistributed around the dawnside or duskside dependent on the direction of By; and (4) the boundary has a general spiral shape, which varies depending on Bz and By.