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in aqueous solution under the visible excitation of up to 488 nm. This nanoprobe exhibits multiple advantages, including long excitation wavelength, high quantum effi ciency, long emission lifetime, narrow emission bands, high photostability, excellent water dispersibility, and good biocompatibility, all of which are very benefi cial for applications in bioimaging. The successful application of nanoprobe in bioimaging with visible excitation has been demonstrated. Thus, the design strategy will be a versatile and convenient way to realize excellent lanthanide(III) complex-based bioprobes for practical biomedical applications. Scheme 1. Design strategy and synthesis procedure of lanthanide(III) complex-based bioporbes sensitized by Ir(III) complexes. 235 wileyonlinelibrary.com