Generally, invertebrates were thought to solely rely on their non-specific innate immune system to fight against invading microorganisms. However, increasing studies have implied that the innate immune response of invertebrates displayed diversity and specificity owing to the hyper-variable immune molecules in organisms. In order to get an insight into the diversity of immune-related genes in Portunus trituberculatus, a full-length transcriptome analysis of several immune-related tissues (hemocytes, hepatopancreas and gills) in P. trituberculatus was performed and the diversity of several immune-related genes was analyzed. The full-length transcriptome analysis of P. trituberculatus was conducted using a combination of SMRT long-read sequencing and Illumina short-read sequencing. A total of 17,433 nonredundant full-length transcripts with average length of 2,271 bp and N50 length of 2,841 bp were obtained, among which 13,978 (80.18%) transcripts were annotated. Moreover, numerous transcript variants of various immune-related genes were identified, including pattern recognition receptors, antimicrobial peptides, heat shock proteins (HSPs), antioxidant enzymes and vital molecules in prophenoloxidase (proPO)-activating system. Based on the full-length transcriptome analysis, open reading frames (ORFs) of four C-type lectins (CTLs) were cloned, and tissue distributions showed that the four CTLs were ubiquitously expressed in all the tested tissues, and mainly expressed in hepatopancreas and gills. The transcription of the four CTLs significantly increased in several immune-related tissues (hemocytes, hepatopancreas and gills) of P. trituberculatus challenged with Vibrio alginolyticus and displayed different profiles. Moreover, the four CTLs displayed distinct bacterial binding and antibacterial activities. The recombinant protein PtCTL-1 (rPtCTL-1) and rPtCTL-3 displayed bacterial binding and antibacterial activities against all tested bacteria. rPtCTL-2 only showed bacterial binding and antibacterial activities against V. alginolyticus. No obvious bacterial binding or antibacterial activities for PtCTL-4 was observed against the tested bacteria. This study enriches the transcriptomic information on P. trituberculatus and provides new insights into the innate immune system of crustaceans. Additionally, our study provided candidates of antibiotic agents for the prevention and treatment of bacteriosis.

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At present, whether the students' dance movements are standard is usually evaluated by subjective methods in the dance teaching process. Therefore, there is often difference in subjective judgment and limitation to human eye observational ability caused by movement velocity. In this paper, the author designed and realized a dance movement scoring system based on gyroscope accelerometer and utilized the multi-node network technology for processing of accelerated speed and angle information collected at various limbs, so as to obtain real-time displacement coordinates and attitude angles. Upon the contrast between curves composed of sequences changing with the time and standard movement curve, the author obtained the overall similarity degree. The experiment has proved that this system is of relatively accurate collection for dance movements. Thus, it can faithfully reflect the movement conditions of limbs in dance movements and provide objective reference data for the evaluation.Key words: gyroscope, multi-node network, displacement, attitude angle, similarity I. PrefaceDance is limb movement with music rhythm. Its basic elements are movement posture, rhythm and expression. The stroking technique of dance has strict regulations in both space and time. It is very difficult to present this complicated three-dimensional spatial movement with simple, direct and understandable figures. Therefore, dance is always deemed as a kind of art which can only be unspeakable. For dance teachers and students in the dance major, it is of great importance for limb movement data in the process of learning, training and performance. This data is helpful for analyzing the presentation conditions of dance movements and skills. However, it is challenging for the issue of how to accurately measure this data. At present, students' dance movements and performances are directly observed and demonstrated by teachers in the teaching process of dance and then evaluated and guided through the method of subjective evaluation. This is taken as a kind of information feedback to help students to improve their movement performance and technical level. This evaluation method will always be influenced by many factors, such as age, hobby, emotion, time and speed of dance movement. Therefore, it is of certain subjectivity, lacking quantitative data foundation.In the human motion capture and analysis system, there is a category of methods based on the International Conference on Education Technology and Economic Management (ICETEM 2015)

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