As a typical plant hormone, indole‐3‐acetic acid (IAA) can regulate the biological activities including division, growth and differentiation of plant cells. In this paper, a MXene and multi‐walled carbon nanotubes composite was prepared by self‐assembly procedure, which was modified on screen‐printed electrode (SPE) to construct a wireless portable electrochemical sensor. Electrochemical investigations of IAA were studied by cyclic voltammetry and an irreversible oxidation process could be observed. The excellent electroanalytical method of IAA was realized on the modified SPE with the detection range as 0.05–125.0 μmol/L and the detection limit as 16.7 nmol/L. The sensor was used for IAA content analysis in different part of pea seedlings with satisfactory results.
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