The development of approaches for the non-thermal sterilization of instruments is an urgent task to ensure the safety of meat industry products, where the use of hot water leads to the formation of condensates and a deterioration in the hygienic condition of the premises. In this study, an installation for sterilizing knives was created, which works by activating aqueous salt solutions with a glow discharge. The power consumption of the installation reactor is only 125–150 Wh. The temperature rise of the sterilizing agent used is about 1.1 ± 0.2 °C/min/L. The effectiveness of the installation for plasma-activation of aqueous solutions of chloride and sodium sulfate by glow discharge (PAW) in relation to the inactivation of microorganisms, including Staphylococcus aureus, Salmonella typhimurium, Pseudomonas gessardii and L. monocytogenes, on steel surfaces was evaluated. Samples of stainless steel (parts of knives) were used in two versions (new and artificially aged). Mono- and polyspecies bacterial biofilms were grown on the surface of the samples. The treatment was carried out by immersing samples of steel plates in plasma-activated aqueous solutions. It was found that the treatment of plates in a knife sterilizer for 1 min had an effective effect on the inhibition of all types of studied bacteria.