Potato proteins (PP) in the lab-made potato juice consists of patatins (38.1%), protease inhibitors (56.7%) and other high molecular weight proteins (5.1%) as characterised by Tricine-SDS-PAGE and MALDI-TOF/TOF-MS. To recover PP from potato juice, the complex behaviours of PP with several natural polyelectrolytes as a function of pH and protein to polyelectrolyte ratio (R PP/Polysaccharide ) were studied by turbidimetric titration. Results indicated that the turbidity curves of PP-polysaccharides displayed a shift to higher pH for chitosan and lower pH for anionic polysaccharides with the decreasing of R PP/Polysaccharide . Chitosan could be used to selectively recover patatin with the purity of 88.6%, and the highest protein yield (51.9%) was achieved at pH 6.0 and R PP/chitosan of 1:2. Carrageenan, one typical anionic polysaccharide, could recover all the PP in potato juice at pH 3.5 and R PP/carrageenan of 2.5:1 with no special protein selectivity.
scite is a Brooklyn-based organization that helps researchers better discover and understand research articles through Smart Citations–citations that display the context of the citation and describe whether the article provides supporting or contrasting evidence. scite is used by students and researchers from around the world and is funded in part by the National Science Foundation and the National Institute on Drug Abuse of the National Institutes of Health.