Introduction:The most cases of cancer death, which are in the first rank among cancers suffered by women is breast cancer. The breast cancer therapy for patients has been done, but still not optimal, so it is necessary to understand the mechanism of therapy in model cell of breast cancer.Aim:This study aim to develop an isolation technique of breast cancer cell from patients as a cancer cell model.Material and methods:Breast cancer cell isolation is performed by enzymatic methods using the collagen I and hyaluronidase. Then, breast cancer cells were characterized using flow cytometry based on the CD44/CD24 expression where MDA-MB468 and MCF-7 breast cancer cell lines were used as positive controls. Estrogen receptor (ER), progesterone receptor (PR), p53, HER2, and Ki67 expression were assessed using an immunohistochemistry assay.Result and Discussion:The morphology of cancer cells was fibroblast like cells on the day 7th after isolation. Isolated breast cancer cells expressed 95.33±0.47% of CD44+/CD24+ and human epidermal growth factor receptor 2 (HER2) low expressions. Isolation of breast cancer cells can use In-house enzymatic protocol. Isolated breast cancer showed the same expression as MDA-MB468 (CD44+/CD24+) and HER2- compared to MCF-7 cell lines (CD44-/CD24+).Conclusion:These cells belonged to a basal type of breast carcinoma and expressed CD44+/CD24+, then isolated BCCs can be used as model cancer cells for further research.

Breast cancer is female most frequent diagnosed cancer and the leading cause of cancer death. The consumption of dietary flavonoids is reported to cause significant breast cancer risk reduction. In vitro studies often used aglycone flavonoids rather than its conjugated form that actually present in human body. Thus its mechanism against breast cancer has not been elucidated completely. The present study aimed to investigate the possible mechanism of dietary flavonoids against breast cancer by in silico study. Conjugated flavonoids were docked to ER (estrogen receptor), HER2 (human epidermal growth factor receptor 2) and EGFR (epidermal growth factor receptor) kinase domains. The molecular docking of 22 flavonoid conjugates towards EGFR and HER2 kinase domain, and ER was successfully performed. Potential binders to proteins: epicatechin conjugates to ER (−8.7 kcal/mol), isoflavone conjugates to HER2 kinase domain (−10.7 kcal/mol), and epigallocatechin and epicatechin conjugates to EGFR kinase domain (−9.2 kcal/mol), were suggested. Supported by other studies, conjugated flavonoids may exert similar inhibitory and agonistic properties to their parent flavonoids. Taken together, the present study showed possible effects of dietary flavonoids against various breast cancer subtypes.

We conclude that the expression of the RAC1, RHOA, and CXCR4 proteins and their interactions play a role as risk factors of NAC infiltration.

Abstract. Breast cancer is a malignancy that occurs in the epithelial tissue of the lobules or ducts of the breast glands. The majority of breast cancer patients come in an advanced stage, which impacts the patient's prognosis. The prognosis of breast cancer can be seen from various prognostic factors. This study aimed to analyze the relationship between tumor size and histopathological gradation with lymph node metastases in breast cancer patients. This cross-sectional study was conducted at Al-Ihsan Hospital, West Java Province on 76 patients. Data obtained from medical records in the form of histopathology lab results taken from January 2020 - December 2020. The data used univariate and bivariate tests. The results of the univariate statistical test showed that the characteristics of breast cancer patients were mostly T3 tumors, histopathological grade 3, and lymph node metastases were negative. The bivariate analysis results showed no relationship between tumor size (P=0.051) and histopathological gradation (P=0.912) with lymph node metastases. Abstrak. Kanker payudara merupakan keganasan yang terjadi pada jaringan epitel lobulus atau duktus dari kelenjar payudara. Mayoritas pasien kanker payudara datang dalam stadium lanjut yang memberikan dampak pada prognosis penderita. Prognosis kanker payudara dapat dilihat dari berbagai faktor prognostik. Tujuan penelitian ini untuk menganalisis hubungan antara ukuran tumor dan gradasi histopatologi dengan metastasis kelenjar getah bening pada penderita kanker payudara. Penelitian cross sectional ini dilakukan di RSUD Al-Ihsan Provinsi Jawa Barat pada 76 pasien. Data diperoleh dari medical record berupa hasil lab histopatologi yang diambil dari Januari 2020 – Desember 2020. Data menggunakan uji univariat dan bivariat Hasil uji statistik univariat didapatkan karakteristik pasien kanker payudara mayoritas berukuran tumor T3, berderajat histopatologi 3, dan metastasis kelenjar getah bening negatif. Hasil analisis bivariat didapatkan tidak terdapat hubungan antara ukuran tumor (P=0,051), dan gradasi histopatologi (P=0,912) dengan metastasis kelenjar getah bening.

Abstract. Breast cancer is a malignant neoplasm disease where in breast tissue and can metastasize. The incidence of breast cancer in Indonesia continues to increase and most of it is found in an advanced stage so that efforts to provide treatment are very difficult and unsatisfactory. This study aims to identify the age characteristics, clinical picture and histopathology of breast cancer. This research is a descriptive observational study. The data obtained came from medical records at the Al-Ihsan Regional Hospital, West Java Province with subjects diagnosed with breast cancer in the January 2018-October 2020 period and then processed using software SPSS. The results of the study were 266 breast cancer patients, with all patients being female, then the largest age range 45-54 years 44.36%, the largest tumor size 2-5 cm 44.74%, histopathological types are most invasive ductal carcinoma 69.92%, grading the highest as grade III 51.13% and lymphovascular invasion with positive 72.18%. It was concluded based characteristic of age, clinical and histopatology description of breast cancer patient in Al-Ihsan Regional Public Hospital of West Java Province January 2018-October 2020 there are 266  breast cancer patients, all women, age range 45-54 years, histopathological type was invasive ductal carcinoma, grading are mostly grade III and lymphovascular invasion are mostly positive. Abstrak. Kanker payudara merupakan suatu penyakit neoplasma yang ganas dimana pada jaringan payudara serta dapat bermetastasis. Insidensi kanker payudara di Indonesia terus meningkat serta kebanyakan ditemukan dalam stadium lanjut sehingga upaya memberikan pengobatan sangat sulit dan tidak memuaskan. Penelitian ini bertujuan untuk mengetahui karakteristik usia, gambaran klinis dan histopatologi kanker payudara. Penelitian ini merupakan penelitian observasional deskriptif. Data yang didapatkan berasal dari rekam medis di RSUD Al-Ihsan Provinsi Jawa Barat dengan subjek yang didiagnosis kanker payudara pada periode Januari 2018-Oktober 2020 kemudian diolah dengan menggunakan software SPSS. Hasil penelitian menunjukan terdapat 266 pasien kanker payudara, dengan semua pasien berjenis kelamin perempuan, kemudian rentang usia terbanyak 45-54 tahun sebesar 44,36%, ukuran tumor terbanyak 2-5 cm 44,74%, jenis histopatologi terbanyak adalah invasive ductal carcinoma 69,92%, grading terbanyak merupakan grade III 51,13% dan invasi limfovaskular dengan hasil positif sebesar 72,18%. Disimpulkan bahwa berdasar atas karakteristik usia, gambaran klinis dan histopatologi pasien kanker payudara di RSUD Al-Ihsan Provinsi Jawa Barat Periode Januari 2018-Oktober 2020 terdapat 266 pasien kanker payudara, semua berjenis kelamin perempuan, rentang usia 45-54 tahun, jenis histopatologi berupa invasive ductal carcinoma, grading terbanyak adalah grade III dan invasi limfovaskular sebagian besar positif.