The word “dermatoglyphics” indicates study of epidermal ridge configuration on palms, soles and fingertips. This investigation was aimed to analyze dermatoglyphic patterns in Klinefelter's syndrome (KS) patients. The study cohort consisted of 57 cases and 25 controls. The prints were taken by using the ink method. Fingertip patterns, triradial counts, a‐t‐d angle and a‐b ridge count were studied. There were significant differences in radial loops and whorls (p<0.05), and there were very highly significant differences in arches (p<0.001) in KS patients as compared to controls. Dermatoglyphic patterns at the hypothenar area (p<0.05), and areas between at the I. interdigital and thenar sites (p<0.001) have been found to be significantly different in KS patients compared to controls. Total ridge counts (TRC), a‐b, a‐t‐d angels were not different in the two groups (p>0.05). A definite correlation between the dermatoglyphic patterns and the KS has been shown.