The effects of Calcium (Ca+2) on virulence and some parameters should be analyzed in this study. Pseudomonas aeruginosa Gram (-) and Bacillus cereus Gram (+) were used. Both bacteria are soil bacteria. In this study; the effect of Ca+2 on protease, amylase, LasB elastolytic assay, H2O2, pyorubin and biofilm on metabolites of these bacteria were investigated during 24 hour time. In this study, the effect of Ca+2 on the production of some secondary metabolites on P. aeruginosa and B. cereus was investigated and presented for the first time by us.

Background: The effects of Ca+2 on virulence and some parameters were tried to be analyzed in this study. P. aeruginosa and B. cereus were used as Gram (-) bacteria as a control and Gram (+) bacteria as a control. Both bacteria are soil bacteria. Results: Amylase; P. aeruginosa is 102% increase in 0.1 M Ca+2 concentrations and B. cereus caused an increase in 123%. Biofilm P. aeruginosa; 200% increase in 0.1 M Ca+2 concentrations. B. cereus caused a 252% increase in the presence of 0.15 M Ca+2. Las B; in accordance with B. cereus, Las B activity decreased 1.7 times more than with P. aeruginosa. H2O2; P. aeruginosa increased by 60% resistance. B. cereus caused only a 3% increase. Protease in P. aeruginosa increase of 220%. B. cereus caused a 548% increase. Pyorubin, a pigment, caused an increase in the presence of Ca+2. However, these increases are not seen as significant increases.Conclusion: In this study; the effect of Ca+2 on protease, amylase, LasB elastolytic assay, H2O2, pyorubin and biofilm was investigated in a 24 hour time. In the study we have conducted here, the effect of Ca+2 meat on the production of some secondary metabolites on Gram (-) P. aeruginosa and Gram (+) B. cereus was investigated for the first time. As a result, although there are many different related studies, they are not comparative as in ours. Therefore, our study has progressed in a different direction and it is a first in its field.