The essential oil, isolated by steam distillation from the aerial parts of Phlomis olivieri Benth. growing wild in Iran, was analysed by GC-MS. Among 15 identified compounds, the major components were germacrene D (28.1%), β-caryophyllene (16.1%), α-pinene (11.7%) and β-selinene (10.2%).

The essential oils of Astrodaucus orientalis (L.) Drude leaves and seeds growing wild in Iran were examined by GC and GC-MS. The composition of the seed oil sample was compared with that of the leaf oil. Twenty-six compounds were identified in both the leaf and the seed oil. Although the composition of the seed oil was similar to that of the leaf oil, quantitative differences in concentration of some constituents were observed. The major components of the leaf oil were fenchyl acetate (44.5%) and α-pinene (21.6%) but the major constituents of the seed oil were myrcene (47.7%) and β-pinene (21.8%). The seed oil was found to contain lower amounts of bornyl acetate, germacrene D and δ-cadinene than the leaf oil.

No abstract

The structure of the glandular trichomes and essential oil of the aerial parts of Marrubium cuneatum Russell were studied. Most secretory tissues are located in glandular trichomes. The essential oil isolated by steam distillation were analysed by GC-MS. Among 25 compounds identified, representing about 89% of the oil, the major components were bicyclogermacrene (37.9%) and germacrene D (24.1% Isolation of the Essential Oil and Preparing SlidesThe essential oil was obtained by water steam distillation of air-dried aerial parts (250 g) in an all-glass apparatus. The sample oil, which was light yellow in colour, was dried over anhydrous sodium sulphate and stored under nitrogen in a sealed vial until required. Fresh stems and leaves were placed in a formalin-acetic acid-ethyl alcohol mixture and slides were prepared by hand-cutting and stained in a alum carmine-methyl green combination (all of the chemicals used were from Merck). They were photographed at standard magnification using an Olympus automatic camera. Gas Chromatography-Mass SpectrometryGC-MS analysis was carried out on a Varian 3400 GC-MS system equipped with a DB-I fused silica column (60 m × 0.25 mm, film thickness 0.25 µm) and interfaced with a Varian ion trap detector. Oven temperature, 50-270°C at a rate of 4°C/min; injector and transfer line temperature, 280°C and 290°C; carrier gas, helium with a linear velocity of 31.5 cm/s; split ratio, 1:60; ionization energy, 70 eV; scan time, 1 s; mass range, 40-400 amu.

No abstract