The paper describes the methods of processing components of distillery bards for fermentation media (and the growth of carbohydrate). There are presented the results of experiments on cultivation in these environments yeast of Phaffia type, the producer of the Supreme carotenoids of astaxanthin

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According to generally accepted international standards, the characterization and confirmation of the quality of biological pharmaceutical substances requires the use of not only a combination of physicochemical and biological tests, but also an in-depth knowledge of the production process and its control. The study of such an industrial process involves considerable expenditure of time and material resources. Gaining a deep knowledge of the production process is greatly facilitated by using laboratory models of industrial installations that effectively reproduce large-scale processes in a research laboratory. On the basis of a laboratory bioreactor for the cultivation of eukaryotic cells with a working volume of 2 L, a scale-down model of a pilot bioreactor has been developed, linearly scalable to a production volume of 1000 L. The laboratory model reproduces the key parameters of cultivation on a pilot scale: the ratio of the geometric dimensions of the reactor, the peripheral speed of the mixer, the specific power input, the coefficient of oxygen mass transfer, the type and intensity of aeration, strategies for defoaming, feeding and maintaining the pH level. Experiments using the developed model showed high similarity in the kinetics of cell growth, productivity and quality of the expressed monoclonal antibodies in laboratory and pilot bioreactors. Key words: cultivation, CHO cells, monoclonal antibodies, scale-down model

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The paper investigates the influence of constant and periodic lighting fluorescent lamps in the range from 0 to 9 500 Lux for the growth of biomass of culture Phaffia rhodozyma and synthesis of astaxanthin at periodic cultivation in the bioreactor. It is established that with the light of 7500 Lux periodic mode of action of light allows culture to synthesize the same astaxanthin as with a constant illumination. It is shown that the constant exposure to the light of 7500 Lux leads to a greater increase in the phase lag of the biosynthesis of astaxanthin and biomass growth than periodic light.